Annika Kröger1,2, Claudia Hülsmann1, Stefan Fickl3, Thomas Spinell4,5, Fabian Hüttig6, Frederic Kaufmann3, André Heimbach7, Per Hoffmann7,8, Norbert Enkling9, Stefan Renvert10, Frank Schwarz11,12, Ryan T Demmer13, Panos N Papapanou14, Søren Jepsen1, Moritz Kebschull1,14,15. 1. Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany. 2. Department of Oral Surgery, School of Dentistry, University of Birmingham, Birmingham, UK. 3. Department of Periodontology, University of Würzburg, Würzburg, Germany. 4. Department of Operative Dentistry and Periodontology, LMU Munich, Munich, Germany. 5. Private Practice, Bolzano, Italy. 6. Department of Prosthodontics, University of Tübingen, Tübingen, Germany. 7. Department of Human Genetics, University of Bonn, Bonn, Germany. 8. Department of Biomedicine, University of Basel, Basel, Switzerland. 9. Department of Prosthodontics, University of Bonn, Bonn, Germany. 10. Department of Health Sciences, Kristianstad University, Kristianstad, Sweden. 11. Department of Oral Surgery and Implantology, Carolinum, Goethe University, Frankfurt, Germany. 12. Department of Oral Surgery, Universitätsklinikum Düsseldorf, Düsseldorf, Germany. 13. Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, Minnesota. 14. Division of Periodontics, Section of Oral, Diagnostic and Rehabilitation Sciences, Columbia University College of Dental Medicine, New York, New York. 15. The School of Dentistry, University of Birmingham, Birmingham, UK.
Abstract
AIM: To cross-sectionally analyse the submucosal microbiome of peri-implantitis (PI) lesions at different severity levels. MATERIALS AND METHODS: Microbial signatures of 45 submucosal plaque samples from untreated PI lesions obtained from 30 non-smoking, systemically healthy subjects were assessed by 16s sequencing. Linear mixed models were used to identify taxa with differential abundance by probing depth, after correction for age, gender, and multiple samples per subject. Network analyses were performed to identify groups of taxa with mutual occurrence or exclusion. Subsequently, the effects of peri-implant probing depth on submucosal microbial dysbiosis were calculated using the microbial dysbiosis index. RESULTS: In total, we identified 337 different taxa in the submucosal microbiome of PI. Total abundance of 12 taxa correlated significantly with increasing probing depth; a significant relationship with lower probing depth was found for 16 taxa. Network analysis identified two mutually exclusive complexes associated with shallow pockets and deeper pockets, respectively. Deeper peri-implant pockets were associated with significantly increased dysbiosis. CONCLUSION: Increases in peri-implant pocket depth are associated with substantial changes in the submucosal microbiome and increasing levels of dysbiosis.
AIM: To cross-sectionally analyse the submucosal microbiome of peri-implantitis (PI) lesions at different severity levels. MATERIALS AND METHODS: Microbial signatures of 45 submucosal plaque samples from untreated PI lesions obtained from 30 non-smoking, systemically healthy subjects were assessed by 16s sequencing. Linear mixed models were used to identify taxa with differential abundance by probing depth, after correction for age, gender, and multiple samples per subject. Network analyses were performed to identify groups of taxa with mutual occurrence or exclusion. Subsequently, the effects of peri-implant probing depth on submucosal microbial dysbiosis were calculated using the microbial dysbiosis index. RESULTS: In total, we identified 337 different taxa in the submucosal microbiome of PI. Total abundance of 12 taxa correlated significantly with increasing probing depth; a significant relationship with lower probing depth was found for 16 taxa. Network analysis identified two mutually exclusive complexes associated with shallow pockets and deeper pockets, respectively. Deeper peri-implant pockets were associated with significantly increased dysbiosis. CONCLUSION: Increases in peri-implant pocket depth are associated with substantial changes in the submucosal microbiome and increasing levels of dysbiosis.
Authors: Philipp Sahrmann; Fabienne Gilli; Daniel B Wiedemeier; Thomas Attin; Patrick R Schmidlin; Lamprini Karygianni Journal: Microorganisms Date: 2020-05-01
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