Ayman Khalil1, Hasan Omran2, Fatima Alsheikh2. 1. Department of Radiation Medicine, Human Nutrition Laboratory, Atomic Energy Commission of Syria (AECS), P.O. Box: 6091, Damascus, Syrian Arab Republic. akhalil@aec.org.sy. 2. Department of Radiation Medicine, Human Nutrition Laboratory, Atomic Energy Commission of Syria (AECS), P.O. Box: 6091, Damascus, Syrian Arab Republic.
Abstract
OBJECTIVE: In this work, the effects of irradiation and high fat diet (HFD) intake have been examined in Wistar rat livers. HFD Wistar rats were exposed three times per week for 2 months to three different doses (0.5, 1, and 2 Gy) of a fractionated whole body gamma irradiation (FWBGI). Hepatic mRNA of these rats was evaluated for five cytokines, TNFα, IL1β, IL6, CRP and IL10. In addition, some critical protein levels were evaluated. RESULTS: Results demonstrated that FWBGI was able to omit the inflammatory state already induced by the HFD through the depression of all pro-inflammatory genes. In addition, TNFα/IL10 IL1β/IL10, IL6/IL10 and CRP/IL10 ratios were less than 1 at all studied irradiation doses. IL6/IL10 ratio (mRNA and protein) was the best that represented an anti-inflammatory state with all used doses. Results could be of great importance in liver radiotherapy in HFD animal models and may give indicators about the inflammatory state improvement during FWBGI.
OBJECTIVE: In this work, the effects of irradiation and high fat diet (HFD) intake have been examined in Wistar rat livers. HFD Wistar rats were exposed three times per week for 2 months to three different doses (0.5, 1, and 2 Gy) of a fractionated whole body gamma irradiation (FWBGI). Hepatic mRNA of these rats was evaluated for five cytokines, TNFα, IL1β, IL6, CRP and IL10. In addition, some critical protein levels were evaluated. RESULTS: Results demonstrated that FWBGI was able to omit the inflammatory state already induced by the HFD through the depression of all pro-inflammatory genes. In addition, TNFα/IL10 IL1β/IL10, IL6/IL10 and CRP/IL10 ratios were less than 1 at all studied irradiation doses. IL6/IL10 ratio (mRNA and protein) was the best that represented an anti-inflammatory state with all used doses. Results could be of great importance in liver radiotherapy in HFD animal models and may give indicators about the inflammatory state improvement during FWBGI.
Authors: Edward E Winger; Jane L Reed; Sherif Ashoush; Tarek El-Toukhy; Sapha Ahuja; Mohamed Taranissi Journal: Am J Reprod Immunol Date: 2011-01-12 Impact factor: 3.886
Authors: Federico Moriconi; Hans Christiansen; Dirk Raddatz; Joszef Dudas; Robert Michael Hermann; Margret Rave-Fränk; Nadeem Sheikh; Bernhard Saile; Clemens Friedrich Hess; Giuliano Ramadori Journal: Radiat Res Date: 2008-02 Impact factor: 2.841