João Pires1, Julia G Kraemer2, Esther Kuenzli3, Sara Kasraian4, Regula Tinguely4, Christoph Hatz3, Andrea Endimiani5, Markus Hilty6. 1. Institute for Infectious Diseases, University of Bern, Bern, Switzerland; Graduate School of Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland. 2. Institute for Infectious Diseases, University of Bern, Bern, Switzerland; Institute for Work and Health (IST), University of Lausanne and University of Geneva, Epalinges, Switzerland. 3. Swiss Tropical and Public Health Institute, Basel, Switzerland; Epidemiology, Biostatistics and Prevention Institute, University of Zurich, Zurich, Switzerland. 4. Institute for Infectious Diseases, University of Bern, Bern, Switzerland. 5. Institute for Infectious Diseases, University of Bern, Bern, Switzerland. Electronic address: andrea.endimiani@ifik.unibe.ch. 6. Institute for Infectious Diseases, University of Bern, Bern, Switzerland. Electronic address: markus.hilty@ifik.unibe.ch.
Abstract
BACKGROUND: Intestinal colonization by extended-spectrum cephalosporin-resistant Enterobacteriaceae (ESC-R-Ent) has been attributed to travel to high prevalence countries. However, the dynamics of the microbiota changes during ESC-R-Ent colonization and whether there is a particular bacterial composition which is associated with subsequent colonization is unknown. METHODS: Forty healthy volunteers living in Switzerland underwent screening before and after a trip to India, and also 3, 6 and 12 months after traveling. Culture-based ESC-R-Ent screening and microbiota analysis based on 16S rRNA amplicon sequencing were performed at all time points. RESULTS: Prevalence of ESC-R-Ent colonization before traveling was 10% (n = 4), whereas it increased to 76% (n = 31) after the trip. Based on bacterial diversity analyses of the gut microbiota, there were few but significant differences for colonized versus non-colonized individuals. However, an alternative, cluster based analysis revealed that individuals remained in the same cluster over time indicating that neither traveling nor ESC-R-Ent colonization significantly influences bacterial composition. Moreover, none of the found microbiota clusters were significantly associated with subsequent risk of ESC-R-Ent colonization. CONCLUSION: Based on their microbiota patterns, every volunteer was at the same risk of ESC-R-Ent colonization while traveling to India. Therefore, other risk factors for ESC-R-Ent colonization are responsible for this phenomenon.
BACKGROUND: Intestinal colonization by extended-spectrum cephalosporin-resistant Enterobacteriaceae (ESC-R-Ent) has been attributed to travel to high prevalence countries. However, the dynamics of the microbiota changes during ESC-R-Ent colonization and whether there is a particular bacterial composition which is associated with subsequent colonization is unknown. METHODS: Forty healthy volunteers living in Switzerland underwent screening before and after a trip to India, and also 3, 6 and 12 months after traveling. Culture-based ESC-R-Ent screening and microbiota analysis based on 16S rRNA amplicon sequencing were performed at all time points. RESULTS: Prevalence of ESC-R-Ent colonization before traveling was 10% (n = 4), whereas it increased to 76% (n = 31) after the trip. Based on bacterial diversity analyses of the gut microbiota, there were few but significant differences for colonized versus non-colonized individuals. However, an alternative, cluster based analysis revealed that individuals remained in the same cluster over time indicating that neither traveling nor ESC-R-Ent colonization significantly influences bacterial composition. Moreover, none of the found microbiota clusters were significantly associated with subsequent risk of ESC-R-Ent colonization. CONCLUSION: Based on their microbiota patterns, every volunteer was at the same risk of ESC-R-Ent colonization while traveling to India. Therefore, other risk factors for ESC-R-Ent colonization are responsible for this phenomenon.
Authors: R Fulchini; W C Albrich; A Kronenberg; A Egli; C R Kahlert; M Schlegel; P Kohler Journal: Epidemiol Infect Date: 2019-08-30 Impact factor: 2.451
Authors: Jan A Stratmann; Raphael Lacko; Olivier Ballo; Shabnam Shaid; Wolfgang Gleiber; Maria J G T Vehreschild; Thomas Wichelhaus; Claudia Reinheimer; Stephan Göttig; Volkhard A J Kempf; Peter Kleine; Susanne Stera; Christian Brandts; Martin Sebastian; Sebastian Koschade Journal: PLoS One Date: 2020-11-25 Impact factor: 3.240
Authors: János Juhász; Balázs Ligeti; Márió Gajdács; Nóra Makra; Eszter Ostorházi; Ferenc Balázs Farkas; Balázs Stercz; Ákos Tóth; Judit Domokos; Sándor Pongor; Dóra Szabó Journal: Antibiotics (Basel) Date: 2021-03-07