Q Tang1, H Ran. 1. Department of Plastic Surgery, Chongqing Three Gorges Central Hospital, Chongqing, China. huiransd@163.com.
Abstract
OBJECTIVE: Wound healing is closely related to the production of inflammatory cytokines, keratinocytes proliferation and migration. This study aims to investigate the role of transmembrane protein 98 (TMEM98) on wound healing and whether miRNA-219-5p could inhibit wound healing by targeting TMEM98 in keratinocytes. MATERIAL AND METHODS: Hypoxia model was established by CoCl2 (2000 μmol/L) treatment. TMEM98 protein expression, inflammatory cytokines (IL-6, IL-8, TNF-α) and cell proliferation and migration were detected in hypoxia group. RESULTS: Overexpression of TMEM98 could significantly reverse the effects caused by hypoxia. MiR-219-5p was markedly increased in hypoxia group and miR-219-5p could downregulate TMEM98 expression by direct binding its 3'-UTR. CONCLUSIONS: We demonstrated that miR-219-5p could inhibit wound healing by targeting TMEM98.
OBJECTIVE: Wound healing is closely related to the production of inflammatory cytokines, keratinocytes proliferation and migration. This study aims to investigate the role of transmembrane protein 98 (TMEM98) on wound healing and whether miRNA-219-5p could inhibit wound healing by targeting TMEM98 in keratinocytes. MATERIAL AND METHODS:Hypoxia model was established by CoCl2 (2000 μmol/L) treatment. TMEM98 protein expression, inflammatory cytokines (IL-6, IL-8, TNF-α) and cell proliferation and migration were detected in hypoxia group. RESULTS: Overexpression of TMEM98 could significantly reverse the effects caused by hypoxia. MiR-219-5p was markedly increased in hypoxia group and miR-219-5p could downregulate TMEM98 expression by direct binding its 3'-UTR. CONCLUSIONS: We demonstrated that miR-219-5p could inhibit wound healing by targeting TMEM98.
Authors: Sally H Cross; Lisa Mckie; Toby W Hurd; Sam Riley; Jimi Wills; Alun R Barnard; Fiona Young; Robert E MacLaren; Ian J Jackson Journal: PLoS Genet Date: 2020-04-01 Impact factor: 5.917