Fatemeh Ghahremani1,2, Amirhosein Kefayat3,4, Daryoush Shahbazi-Gahrouei2, Hasan Motaghi5, Masoud A Mehrgardi5, Shaghayegh Haghjooy-Javanmard6. 1. Department of Medical Physics & Radiotherapy, Arak University of Medical Sciences, Arak 38481-76941, Iran. 2. Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran. 3. Department of Oncology, Cancer Prevention Research Center, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran. 4. Department of Oncology, Seyed Al-Shohada Hospital, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran. 5. Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran. 6. Department of Physiology, Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan 81746-73461, Iran.
Abstract
AIM: Herein, the AS1411 aptamer-targeted ultrasmall gold nanoclusters (GNCs) were assessed at different aspects as a radiosensitizer. MATERIALS & METHODS: AS1411 aptamer-conjugated gold nanoclusters (Apt-GNCs) efficacy was evaluated at cancer cells targeting, radiosensitizing effect, tumor targeting, and biocompatibility in breast tumor-bearing mice. RESULTS: Flow cytometry and fluorescence microscopy exhibited more cellular uptake for Apt-GNCs in comparison with GNCs. In addition, inductively coupled plasma optical emission spectrometry results demonstrated its effective tumor targeting as the tumors' gold content for GNCs and Apt-GNCs were 8.53 and 15.33 μg/g, respectively. Apt-GNCs significantly enhanced radiotherapy efficacy as mean tumors' volume decreased about 39% and 9 days increase in the mice survival was observed. Both GNCs and Apt-GNCs were biocompatible. CONCLUSION: The Apt-GNCs is a novel and efficient radiosensitizer.
AIM: Herein, the AS1411 aptamer-targeted ultrasmall gold nanoclusters (GNCs) were assessed at different aspects as a radiosensitizer. MATERIALS & METHODS: AS1411 aptamer-conjugated gold nanoclusters (Apt-GNCs) efficacy was evaluated at cancer cells targeting, radiosensitizing effect, tumor targeting, and biocompatibility in breast tumor-bearing mice. RESULTS: Flow cytometry and fluorescence microscopy exhibited more cellular uptake for Apt-GNCs in comparison with GNCs. In addition, inductively coupled plasma optical emission spectrometry results demonstrated its effective tumor targeting as the tumors' gold content for GNCs and Apt-GNCs were 8.53 and 15.33 μg/g, respectively. Apt-GNCs significantly enhanced radiotherapy efficacy as mean tumors' volume decreased about 39% and 9 days increase in the mice survival was observed. Both GNCs and Apt-GNCs were biocompatible. CONCLUSION: The Apt-GNCs is a novel and efficient radiosensitizer.
Authors: Gema Vivo-Llorca; Ángela Morellá-Aucejo; Alba García-Fernández; Paula Díez; Antoni Llopis-Lorente; Mar Orzáez; Ramón Martínez-Máñez Journal: Int J Nanomedicine Date: 2022-01-26