Literature DB >> 3033062

Double-immunolabeling systems for phenotyping of immune cells harboring bovine viral diarrhea virus.

H Bielefeldt Ohmann.   

Abstract

Optimal staining conditions were defined for simultaneous detection of bovine viral diarrhea virus (BVDV) and mononuclear leukocyte surface antigens in tissue sections and cytospins. Because of the extreme lability of the virus antigens and the variable stability of the epitopes on the cell differentiation antigens, cryopreservation had to be used. This method gives slightly sub-optimal preservation of morphology. However, the specificity and sensitivity of the immunolabeling ensured reliable identification of the double-labeled cells, i.e., the phenotypic identification of virus-infected cells within the immune system.

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Year:  1987        PMID： 3033062     DOI： 10.1177/35.6.3033062

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  10 in total

1.  BVD virus antigens in tissues of persistently viraemic, clinically normal cattle: implications for the pathogenesis of clinically fatal disease.

Authors:  H Bielefeldt Ohmann
Journal:  Acta Vet Scand       Date:  1988       Impact factor: 1.695

2.  Neuro-invasion by a 'Trojan Horse' strategy and vasculopathy during intrauterine flavivirus infection.

Authors:  Helle Bielefeldt-Ohmann; Natalia P Smirnova; Airn-Elizabeth Tolnay; Brett T Webb; Alfredo Q Antoniazzi; Hana van Campen; Thomas R Hansen
Journal:  Int J Exp Pathol       Date:  2012-02       Impact factor: 1.925

3.  Monoclonal antibodies to the p80/125 gp53 proteins of bovine viral diarrhea virus: their potential use as diagnostic reagents.

Authors:  D Deregt; S A Masri; H J Cho; H Bielefeldt Ohmann
Journal:  Can J Vet Res       Date:  1990-06       Impact factor: 1.310

4.  Cell phenotypes in the efferent lymph of sheep persistently infected with Border disease virus.

Authors:  G Entrican; J Hopkins; M Maclean; I McConnell; P F Nettleton
Journal:  Clin Exp Immunol       Date:  1992-03       Impact factor: 4.330

5.  The spike glycoprotein of murine coronavirus MHV-JHM mediates receptor-independent infection and spread in the central nervous systems of Ceacam1a-/- Mice.

Authors:  Tanya A Miura; Emily A Travanty; Lauren Oko; Helle Bielefeldt-Ohmann; Susan R Weiss; Nicole Beauchemin; Kathryn V Holmes
Journal:  J Virol       Date:  2007-11-14       Impact factor: 5.103

6.  Immunofluorescence of bovine virus diarrhea viral antigen in white blood cells from experimentally infected immunocompetent calves.

Authors:  D M Bezek; J C Baker; J B Kaneene
Journal:  Can J Vet Res       Date:  1988-04       Impact factor: 1.310

7.  Comparative serological response in calves to eight commercial vaccines against infectious bovine rhinotracheitis, parainfluenza-3, bovine respiratory syncytial, and bovine viral diarrhea viruses.

Authors:  J Van Donkersgoed; J V van den Hurk; D McCartney; R J Harland
Journal:  Can Vet J       Date:  1991-12       Impact factor: 1.008

8.  Patho- and immunobiology of malignant mesothelioma: characterisation of tumour infiltrating leucocytes and cytokine production in a murine model.

Authors:  H Bielefeldt-Ohmann; D R Fitzpatrick; A L Marzo; A G Jarnicki; R P Himbeck; M R Davis; L S Manning; B W Robinson
Journal:  Cancer Immunol Immunother       Date:  1994-12       Impact factor: 6.968

9.  Bovine herpesvirus-1-induced pharyngeal tonsil lesions in neonatal and weanling calves.

Authors:  J C Schuh; H Bielefeldt Ohmann; L A Babiuk; C E Doige
Journal:  J Comp Pathol       Date:  1992-04       Impact factor: 1.311

Review 10.  The pathologies of bovine viral diarrhea virus infection. A window on the pathogenesis.

Authors:  H Bielefeldt-Ohmann
Journal:  Vet Clin North Am Food Anim Pract       Date:  1995-11       Impact factor: 3.357
  10 in total

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