Ítalo Medeiros Azevedo1, Irami Araújo-Filho2, Marianny Maiara Antas Teixeira3, Marília Daniela Ferreira de Carvalho Moreira4, Aldo Cunha Medeiros5. 1. Fellow PhD degree, Postgraduate Program in Health Sciences, Universidade Federal do Rio Grande do Norte (UFRN), Natal-RN, Brazil. Acquisition and interpretation of data, technical procedures, manuscript preparation. 2. PhD, Assistant Professor, Department of Surgery, UFRN, Natal-RN, Brazil. Technical procedures, manuscript preparation. 3. Graduate student, UFRN, Natal-RN, Brazil. Technical procedures, manuscript preparation. 4. PhD, Hospital Universitário Onofre Lopes, UFRN, Natal-RN, Brazil. Technical procedures, manuscript preparation. 5. Full Professor, Chairman, Nucleus of Experimental Surgery, UFRN, Natal-RN, Brazil. Conception, design, intellectual and scientific content of the study; critical revision.
Abstract
PURPOSE: To evaluate if Moringa oleifera leaf aqueous extract (ME) influences the healing of skin wounds of diabetic rats. METHODS: Wistar rats were used (6 rats/group). Group 1 received normal saline (NS) v.o. Group 2 received moringa extract (100mg/kg v.o) for 3 weeks. Groups 3 and 4: Streptozotocin (STZ) induced diabetes. Group 3 received NS; Group 4 received aqueous ME (100mg/kg) v.o.The wounds of groups 1 and 3 rats were topically treated with NS; wounds of groups 2 and 4 treated with 200µL of 10% ME. After anesthesia, all rats had skin square excision wounds 1.5cm2. Wound percent contractions were measured. On 10th day, blood glucose and serum cytokines were measured. Histometry of wounds was studied using ImagePro6.0 software. RESULTS: Glycemia was significantly reduced in ME treated rats. These rats had higher percent contraction of the wounds on 2nd, 5th and 10th days, then controls (p<0.05). Diabetic rats treated with NS had TNF-α, IL-1β and IL-6 expression higher than in rats receiving ME. The histopathological score of ME treated diabetic rats (198±13.7) was significantly higher than treatment with NS (145±10.5). CONCLUSION: ME extract positively influenced healing of wounds in diabetic rats after systemic and topical treatment.
PURPOSE: To evaluate if Moringa oleifera leaf aqueous extract (ME) influences the healing of skin wounds of diabeticrats. METHODS:Wistar rats were used (6 rats/group). Group 1 received normal saline (NS) v.o. Group 2 received moringa extract (100mg/kg v.o) for 3 weeks. Groups 3 and 4: Streptozotocin (STZ) induced diabetes. Group 3 received NS; Group 4 received aqueous ME (100mg/kg) v.o.The wounds of groups 1 and 3 rats were topically treated with NS; wounds of groups 2 and 4 treated with 200µL of 10% ME. After anesthesia, all rats had skin square excision wounds 1.5cm2. Wound percent contractions were measured. On 10th day, blood glucose and serum cytokines were measured. Histometry of wounds was studied using ImagePro6.0 software. RESULTS: Glycemia was significantly reduced in ME treated rats. These rats had higher percent contraction of the wounds on 2nd, 5th and 10th days, then controls (p<0.05). Diabeticrats treated with NS had TNF-α, IL-1β and IL-6 expression higher than in rats receiving ME. The histopathological score of ME treated diabeticrats (198±13.7) was significantly higher than treatment with NS (145±10.5). CONCLUSION: ME extract positively influenced healing of wounds in diabeticrats after systemic and topical treatment.
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