Yashpal S Chhonker1, Liping Ma2, Constant Edi3, Daryl J Murry1,4. 1. Department of Pharmacy Practice, University of Nebraska Medical Center, Omaha, NE 68198, USA. 2. Office of Clinical Trial Institution & Department of Pharmacy, Peking University Shougang Hospital, No 10 Jing-yuan Road, Jinyuanzhuang, Shijingshan District, Beijing100144, PR China. 3. Centre Suisse de Recherches Scientifiques en Côte d'Ivoire (CSRS), 01 BP1303 Abidjan 01, Côte d'Ivoire, West Africa. 4. Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Abstract
AIM: A sensitive and selective LC-MS/MS method was validated for quantitation of ivermectin (IVM) in plasma. METHOD: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1:1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode. RESULTS: The MS/MS response was linear over the concentration range from 0.1-1000 ng/ml. The method for human plasma was validated as per US FDA guidelines. The LC-MS/MS method is sensitive, reproducible, has easy sample preparation and is suitable for IVM quantitation in clinical samples.
AIM: A sensitive and selective LC-MS/MS method was validated for quantitation of ivermectin (IVM) in plasma. METHOD: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1:1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode. RESULTS: The MS/MS response was linear over the concentration range from 0.1-1000 ng/ml. The method for human plasma was validated as per US FDA guidelines. The LC-MS/MS method is sensitive, reproducible, has easy sample preparation and is suitable for IVM quantitation in clinical samples.
Authors: Francisco M Marty; Colleen M Lowry; Martin Rodriguez; Danny A Milner; Walter S Pieciak; Anushua Sinha; Lawrence Fleckenstein; Lindsey R Baden Journal: Clin Infect Dis Date: 2005-05-11 Impact factor: 9.079
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