Literature DB >> 30324453

Noninvasive Two-Photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium.

Grazyna Palczewska1, Timothy S Kern2, Krzysztof Palczewski3.   

Abstract

Two-photon excitation microscopy is perfectly suited for imaging deep into the retina due to its use of infrared (IR) wavelengths to excite endogenous fluorophores such as vitamin A-derived retinoids present in this tissue. Furthermore, two-photon excitation occurs only around a small focal volume, and scattered IR photons cannot excite retinal chromophores. These characteristics contribute to subcellular resolution and low noise of images obtained from deep within retinal layers. Here we describe how to customize a two-photon microscope for noninvasive imaging of the retina and retinal pigment epithelium (RPE) in the mouse eye, along with detailed instructions for mouse handling and retinal imaging, and we provide examples of mouse retinal two-photon microscopy data.

Entities:  

Keywords:  Mouse eye; RPE; Retina; Two-photon microscopy; Vitamin A

Mesh：

Substances：

Year:  2019        PMID： 30324453      PMCID： PMC6991118          DOI： 10.1007/978-1-4939-8669-9_21

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

1.  Two-photon absorption and the design of two-photon dyes.

Authors:  Miłosz Pawlicki; Hazel A Collins; Robert G Denning; Harry L Anderson
Journal:  Angew Chem Int Ed Engl       Date:  2009       Impact factor: 15.336

2.  In vivo two-photon imaging of the mouse retina.

Authors:  Robin Sharma; Lu Yin; Ying Geng; William H Merigan; Grazyna Palczewska; Krzysztof Palczewski; David R Williams; Jennifer J Hunter
Journal:  Biomed Opt Express       Date:  2013-07-09       Impact factor: 3.732

3.  Two-photon laser scanning fluorescence microscopy.

Authors:  W Denk; J H Strickler; W W Webb
Journal:  Science       Date:  1990-04-06       Impact factor: 47.728

4.  Human infrared vision is triggered by two-photon chromophore isomerization.

Authors:  Grazyna Palczewska; Frans Vinberg; Patrycjusz Stremplewski; Martin P Bircher; David Salom; Katarzyna Komar; Jianye Zhang; Michele Cascella; Maciej Wojtkowski; Vladimir J Kefalov; Krzysztof Palczewski
Journal:  Proc Natl Acad Sci U S A       Date:  2014-12-01       Impact factor: 11.205

5.  Two-photon microscopy reveals early rod photoreceptor cell damage in light-exposed mutant mice.

Authors:  Akiko Maeda; Grazyna Palczewska; Marcin Golczak; Hideo Kohno; Zhiqian Dong; Tadao Maeda; Krzysztof Palczewski
Journal:  Proc Natl Acad Sci U S A       Date:  2014-03-24       Impact factor: 11.205

6.  Noninvasive multiphoton fluorescence microscopy resolves retinol and retinal condensation products in mouse eyes.

Authors:  Grazyna Palczewska; Tadao Maeda; Yoshikazu Imanishi; Wenyu Sun; Yu Chen; David R Williams; David W Piston; Akiko Maeda; Krzysztof Palczewski
Journal:  Nat Med       Date:  2010-11-14       Impact factor: 53.440

7.  Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging.

Authors:  Michelle Cua; Daniel J Wahl; Yuan Zhao; Sujin Lee; Stefano Bonora; Robert J Zawadzki; Yifan Jian; Marinko V Sarunic
Journal:  Sci Rep       Date:  2016-09-07       Impact factor: 4.379

8.  Standards for reporting the optical aberrations of eyes.

Authors:  Larry N Thibos; Raymond A Applegate; James T Schwiegerling; Robert Webb
Journal:  J Refract Surg       Date:  2002 Sep-Oct       Impact factor: 3.573

Review 9.  Retinosomes: new insights into intracellular managing of hydrophobic substances in lipid bodies.

Authors:  Yoshikazu Imanishi; Volker Gerke; Krzysztof Palczewski
Journal:  J Cell Biol       Date:  2004-08-16       Impact factor: 10.539

10.  Physiological and microfluorometric studies of reduction and clearance of retinal in bleached rod photoreceptors.

Authors:  Efthymia Tsina; Chunhe Chen; Yiannis Koutalos; Petri Ala-Laurila; Marco Tsacopoulos; Barbara Wiggert; Rosalie K Crouch; M Carter Cornwall
Journal:  J Gen Physiol       Date:  2004-10       Impact factor: 4.086
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  3 in total

1.  Sensitivity of Mammalian Cone Photoreceptors to Infrared Light.

Authors:  Frans Vinberg; Grazyna Palczewska; Jianye Zhang; Katarzyna Komar; Maciej Wojtkowski; Vladimir J Kefalov; Krzysztof Palczewski
Journal:  Neuroscience       Date:  2019-08-07       Impact factor: 3.590

2.  Detailed 3D micro-modeling of rat aqueous drainage channels based on two-photon imaging: simulating aqueous humor through trabecular meshwork and Schlemm's canal by two-way fluid structure interaction approach.

Authors:  Jing Zhang; Xiuqing Qian; Haixia Zhang; Huanhuan Chu; Hang Xu; Zhicheng Liu
Journal:  Med Biol Eng Comput       Date:  2022-05-06       Impact factor: 2.602

3.  Transpupillary Two-photon In vivo Imaging of the Mouse Retina.

Authors:  Zelun Wang; Sean McCracken; Philip R Williams
Journal:  J Vis Exp       Date:  2021-02-13       Impact factor: 1.424
  3 in total

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