PURPOSE: To evaluate the regenerative potential of surnatants (SNs) from bone marrow concentrate (SN-BMC) and expanded mesenchymal stromal cells (SN-MSCs) loaded onto a collagen scaffold (SC) in comparison with cell-based treatments (BMC and MSCs) in an osteochondral (OC) defect model in rabbits. METHODS: OC defects (3 × 5 mm) were created in the rabbit femoral condyles and treated with SC alone or combined with SN-BMC, SN-MSCs, BMC, and MSCs. In control groups, the defects were left untreated. At three and six months, the quality of regenerated tissue was evaluated with macroscopic, histologic, microtomographic, and immunohistochemical assessments. The production of several immunoenzymatic markers was measured in the synovial fluid. RESULTS: All proposed treatments improved OC regeneration in comparison with untreated and SC-treated defects. Both BMC and MSCs showed a similar healing potential than their respective SNs, with the best performance exerted by BMC as demonstrated with macroscopic and histological scores and type I and II collagen results. CONCLUSIONS: SNs loaded onto SC exerted a positive effect on OC defect regeneration, underlying the biological significance of the trophic factors, thus potentially opening new opportunities for the use of cell-free-based therapies. BMC was confirmed to be the most beneficial treatment.
PURPOSE: To evaluate the regenerative potential of surnatants (SNs) from bone marrow concentrate (SN-BMC) and expanded mesenchymal stromal cells (SN-MSCs) loaded onto a collagen scaffold (SC) in comparison with cell-based treatments (BMC and MSCs) in an osteochondral (OC) defect model in rabbits. METHODS: OC defects (3 × 5 mm) were created in the rabbit femoral condyles and treated with SC alone or combined with SN-BMC, SN-MSCs, BMC, and MSCs. In control groups, the defects were left untreated. At three and six months, the quality of regenerated tissue was evaluated with macroscopic, histologic, microtomographic, and immunohistochemical assessments. The production of several immunoenzymatic markers was measured in the synovial fluid. RESULTS: All proposed treatments improved OC regeneration in comparison with untreated and SC-treated defects. Both BMC and MSCs showed a similar healing potential than their respective SNs, with the best performance exerted by BMC as demonstrated with macroscopic and histological scores and type I and II collagen results. CONCLUSIONS: SNs loaded onto SC exerted a positive effect on OC defect regeneration, underlying the biological significance of the trophic factors, thus potentially opening new opportunities for the use of cell-free-based therapies. BMC was confirmed to be the most beneficial treatment.
Entities:
Keywords:
Bone marrow concentrate; Cell-free approach; In vivo model; Mesenchymal stromal cells; Osteochondral defect
Authors: Maryam Tamaddon; Gordon Blunn; Wei Xu; Maria Elena Alemán Domínguez; Mario Monzón; James Donaldson; John Skinner; Timothy R Arnett; Ling Wang; Chaozong Liu Journal: Bone Joint Res Date: 2021-10 Impact factor: 5.853
Authors: Chengchong Ai; Yee Han Dave Lee; Xuan Hao Tan; Si Heng Sharon Tan; James Hoi Po Hui; James Cho-Hong Goh Journal: J Orthop Translat Date: 2021-10-11 Impact factor: 5.191
Authors: Deepak V Chona; Stephanie T Kha; Paul D Minetos; Christopher M LaPrade; Constance R Chu; Geoffrey D Abrams; Marc R Safran; Seth L Sherman Journal: Orthop J Sports Med Date: 2021-11-04