BACKGROUND: Inflammatory bowel diseases (IBDs), which include ulcerative colitis (UC) and Crohn's disease (CD), are inflammatory disorders that affect the gastrointestinal tract. A combination of inflammatory cytokines has an important role in IBD development. Genome-wide association studies have shown that polymorphisms in the interleukin-23R gene (IL-23R) increase susceptibility to IBD. The aim of this study was to investigate the IL-23R 3' UTR SNP to determine a potential association between genotype distribution and IBD. METHODS: The case group included 102 IBD patients and the control group included 107 healthy individuals. IL-23R polymorphisms rs10889677 were genotyped using PCR-RFLP analysis. RFLP results were confirmed by direct sequencing. RESULTS: The allele and genotype frequencies in patients and controls were evaluated and compared, and no significant association between this functional rs10889677 polymorphism and risk of IBD was observed (P=0.587; adjusted OR: 0.89; 95% CI: 0.597-1.339). We also found no significant association between CD (14.71%) and UC (85.29%) patients in allele or genotype levels (P>0.05). CONCLUSION: Our results suggest that the rs10889677 A>C polymorphism is not a potential prognostic marker in Iranian patients with IBD.
BACKGROUND: Inflammatory bowel diseases (IBDs), which include ulcerative colitis (UC) and Crohn's disease (CD), are inflammatory disorders that affect the gastrointestinal tract. A combination of inflammatory cytokines has an important role in IBD development. Genome-wide association studies have shown that polymorphisms in the interleukin-23R gene (IL-23R) increase susceptibility to IBD. The aim of this study was to investigate the IL-23R 3' UTR SNP to determine a potential association between genotype distribution and IBD. METHODS: The case group included 102 IBD patients and the control group included 107 healthy individuals. IL-23R polymorphisms rs10889677 were genotyped using PCR-RFLP analysis. RFLP results were confirmed by direct sequencing. RESULTS: The allele and genotype frequencies in patients and controls were evaluated and compared, and no significant association between this functional rs10889677 polymorphism and risk of IBD was observed (P=0.587; adjusted OR: 0.89; 95% CI: 0.597-1.339). We also found no significant association between CD (14.71%) and UC (85.29%) patients in allele or genotype levels (P>0.05). CONCLUSION: Our results suggest that the rs10889677 A>C polymorphism is not a potential prognostic marker in Iranian patients with IBD.
Authors: Antonie Zwiers; Laurens Kraal; Tineke C T M van de Pouw Kraan; Thomas Wurdinger; Gerd Bouma; Georg Kraal Journal: J Immunol Date: 2012-01-18 Impact factor: 5.422
Authors: Richard H Duerr; Kent D Taylor; Steven R Brant; John D Rioux; Mark S Silverberg; Mark J Daly; A Hillary Steinhart; Clara Abraham; Miguel Regueiro; Anne Griffiths; Themistocles Dassopoulos; Alain Bitton; Huiying Yang; Stephan Targan; Lisa Wu Datta; Emily O Kistner; L Philip Schumm; Annette T Lee; Peter K Gregersen; M Michael Barmada; Jerome I Rotter; Dan L Nicolae; Judy H Cho Journal: Science Date: 2006-10-26 Impact factor: 47.728
Authors: Toshihiko Okazaki; Ming-Hsi Wang; Patricia Rawsthorne; Michael Sargent; Lisa Wu Datta; Yin Yao Shugart; Charles N Bernstein; Steven R Brant Journal: Inflamm Bowel Dis Date: 2008-11 Impact factor: 5.325
Authors: Siew C Ng; Wai Keung Leung; Hai Yun Shi; Michael K K Li; Chi Man Leung; Carmen K M Ng; Fu Hang Lo; Yee Tak Hui; Steven W C Tsang; Yiu Kay Chan; Ching Kong Loo; Kam Hon Chan; Aric J Hui; Wai Hung Chow; Marcus Harbord; Jessica Y L Ching; Mandy Lee; Victor Chan; Whitney Tang; Ivan F N Hung; Judy Ho; Wai Cheung Lao; Marc T L Wong; Shun Fung Sze; Edwin H S Shan; Belsy C Y Lam; Raymond W H Tong; Lai Yee Mak; Sai Ho Wong; Justin C Y Wu; Francis K L Chan; Joseph J Y Sung Journal: Inflamm Bowel Dis Date: 2016-08 Impact factor: 5.325
Authors: Thomas Welte; Samuel S M Zhang; Tian Wang; Zhiyuan Zhang; David G T Hesslein; Zhinan Yin; Arihiro Kano; Yoshiki Iwamoto; En Li; Joseph E Craft; Alfred L M Bothwell; Erol Fikrig; Pandelakis A Koni; Richard A Flavell; Xin-Yuan Fu Journal: Proc Natl Acad Sci U S A Date: 2003-02-05 Impact factor: 11.205
Authors: Vahid Chaleshi; Raziye Tajali; Sanaz Savabkar; Neda Zali; Ehsan N Mojarad; Mehrdad Haghazali; Sara Pasha; Hamid A Aghdaei; Mohammad R Zali; Mohsen Vahedi Journal: Microrna Date: 2016
Authors: Heike Schmitt; Ulrike Billmeier; Walburga Dieterich; Timo Rath; Sophia Sonnewald; Stephen Reid; Simon Hirschmann; Kai Hildner; Maximilian J Waldner; Jonas Mudter; Arndt Hartmann; Robert Grützmann; Clemens Neufert; Tino Münster; Markus F Neurath; Raja Atreya Journal: Gut Date: 2018-05-30 Impact factor: 23.059