Mojtaba Saffari1, Seyyed Mohammad Hossein Ghaderian2, Mir Davood Omrani3, Mandana Afsharpad4, Kimia Shankaie5, Niusha Samadaian6. 1. Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. m-saffari@sbmu.ac.ir. 2. Chronic Kidney Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 4. Cancer Control Research Center, Cancer Control Fundation, Iran, Iran University of Medical Sciences, Tehran, Iran. 5. Department of Biology, Science and research branch, Islamic Azad University, Tehran, Iran. 6. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
PURPOSE: This study aims to investigate the expression level of mir-let7b-3p and mir-548, which are involved in PTEN expression in tissue samples of prostate cancer patients versus benign prostate hyperplasia (BPH) and normal adjacent tissue. MATERIALS AND METHODS: Prostate cancer tissues were obtained from patients after receiving informed consent. Total RNA extraction and cDNA synthesis were performed for determining gene expression. RESULTS: Ten patients were determined to have high Gleason scores (> 7), 36 and seven samples had intermediate Gleason scores (7?) and BPH, respectively, and 40 samples were derived from normal adjacent tissue. Downreg-ulation of mir-let7b and upregulation of mir-548 expression significantly correlated with high-risk Gleason scores. CONCLUSION: The present study showed that miR-let7b and/or mir-548 can be considered as potential targets in prostate cancer therapy.
PURPOSE: This study aims to investigate the expression level of mir-let7b-3p and mir-548, which are involved in PTEN expression in tissue samples of prostate cancerpatients versus benign prostate hyperplasia (BPH) and normal adjacent tissue. MATERIALS AND METHODS:Prostate cancer tissues were obtained from patients after receiving informed consent. Total RNA extraction and cDNA synthesis were performed for determining gene expression. RESULTS: Ten patients were determined to have high Gleason scores (> 7), 36 and seven samples had intermediate Gleason scores (7?) and BPH, respectively, and 40 samples were derived from normal adjacent tissue. Downreg-ulation of mir-let7b and upregulation of mir-548 expression significantly correlated with high-risk Gleason scores. CONCLUSION: The present study showed that miR-let7b and/or mir-548 can be considered as potential targets in prostate cancer therapy.