| Literature DB >> 30317509 |
Haijia Yu1,2, Lauren Crisman3, Michael H B Stowell3, Jingshi Shen4.
Abstract
The fusion of intracellular vesicles with target membranes is mediated by two classes of conserved molecules-soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAP receptors or SNAREs) and Sec1/Munc18 (SM) proteins. A conserved function of SM proteins is to recognize their cognate trans-SNARE complexes and accelerate fusion kinetics. Here, we describe a physiologically relevant reconstitution system in which macromolecular crowding agents are included to recapitulate the crowded intracellular environment. Through this system, we elucidate the molecular mechanisms by which SNAREs and SM proteins drive vesicle fusion.Entities:
Keywords: Content mixing; Lipid mixing; Macromolecular crowding; Membrane fusion; Reconstitution; SM protein; SNARE; Vesicle fusion
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Year: 2019 PMID： 30317509 PMCID： PMC6869331 DOI： 10.1007/978-1-4939-8760-3_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745