| Literature DB >> 30317508 |
Ekaterina Stroeva1, Shyam S Krishnakumar2,3.
Abstract
In this chapter, we introduce a nanodisc-based experimental platform to study Ca2+-triggered membrane interaction of synaptotagmin-1. We describe and discuss in detail how to assemble this soluble mimetic of the docked vesicle-plasma membrane junction, with fluorescently labeled synaptotagmin-1 bound to trans SNAREpins assembled between nanodiscs and present the stopped-flow rapid mixing method used to monitor the conformational dynamics of Ca2+-activation process on a millisecond timescale.Entities:
Keywords: Calcium activation; Fluorescent labeling; Membrane interaction; Nanodiscs; Rapid mixing stopped-flow; SNARE proteins; Soluble membrane-mimetic; Synaptotagmin-1
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Year: 2019 PMID： 30317508 DOI： 10.1007/978-1-4939-8760-3_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745