| Literature DB >> 30317498 |
Byoungjae Kong1, Yoosoo Yang2,3, Dae-Hyuk Kweon4,5.
Abstract
Dynamic light scattering (DLS) spectroscopy provides rapid information on the size distribution of a large number of particles in a mixture. Vesicle sizes change during the merger of lipid bilayers, and DLS analysis can provide rapid, accurate, and non-perturbative quantification of the size distribution of proteoliposomes in SNARE-dependent membrane fusion. In this chapter, we describe the methodologies and reagents used for DLS spectroscopy in a biochemical and biophysical study of SNARE-mediated membrane fusion.Entities:
Keywords: Dynamic light scattering; Membrane fusion; SNARE; Size distribution
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Year: 2019 PMID： 30317498 DOI： 10.1007/978-1-4939-8760-3_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745