Jerry R Paugh1, Alba Alfonso-Garcia2, Andrew Loc Nguyen3, Jeffrey L Suhalim4, Marjan Farid5, Sumit Garg5, Jeremiah Tao5, Donald J Brown5, Eric O Potma6, James V Jester7. 1. Southern California College of Optometry at Marshall B. Ketchum University, Fullerton, CA, USA. Electronic address: jpaugh@ketchum.edu. 2. Department of Chemistry, University of California, Irvine, Irvine, CA, USA; University of California at Davis, Department of Biomedical Engineering, Davis, CA, USA. 3. Department of Mathematics, California State University Fullerton, California, USA. 4. Department of Biomedical Engineering, University of California, Irvine, CA, USA. 5. Gavin Herbert Eye Institute, University of California, Irvine, CA, USA. 6. Department of Chemistry, University of California, Irvine, Irvine, CA, USA. 7. Department of Biomedical Engineering, University of California, Irvine, CA, USA; Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
Abstract
PURPOSE: This study examined whether hyperspectral stimulated Raman scattering (hsSRS) microscopy can detect differences in meibum lipid to protein composition of normal and evaporative dry eye subjects with meibomian gland dysfunction. METHODS: Subjects were evaluated for tear breakup time (TBUT), staining, meibum expression and gland dropout. Expressed meibum was analyzed using SRS vibrational signatures in the CH stretching region (2800-3050 cm-1). Vertex component analysis and K-means clustering were used to group the spectral signatures into four fractions containing high lipid (G1) to high protein (G4). RESULTS: Thirty-three subjects could be statistically analyzed using pooled meibum (13 with stable tear films (TBUTs > 10 s) and 20 with unstable tear films (TBUTs ≤ 10 s). Significant differences in meibum from subjects with unstable vs. stable TBUTs were found for the G1 fraction (medians 0.164 and 0.020, respectively; p = 0.012) and the G2 fraction (medians 0.244 and 0.272, respectively; p = 0.045). No differences were observed for the G3 and G4 fractions. Single orifice samples were not significantly different vs. pooled samples from the fellow eye, and eyelid sector samples (nasal, central and temporal) G2:G3 fractional components were not significantly different (p = 0.449). Spearman analysis suggested a significant inverse correlation between G1 fraction and TBUT (R = -0.351; p = 0.045). CONCLUSIONS: hsSRS microscopy allows compositional analysis of expressed meibum from humans which correlated to changes in TBUT. These findings support the hypothesis that hsSRS may be useful in classifying meibum quality and evaluating the effects of therapy.
PURPOSE: This study examined whether hyperspectral stimulated Raman scattering (hsSRS) microscopy can detect differences in meibum lipid to protein composition of normal and evaporative dry eye subjects with meibomian gland dysfunction. METHODS: Subjects were evaluated for tear breakup time (TBUT), staining, meibum expression and gland dropout. Expressed meibum was analyzed using SRS vibrational signatures in the CH stretching region (2800-3050 cm-1). Vertex component analysis and K-means clustering were used to group the spectral signatures into four fractions containing high lipid (G1) to high protein (G4). RESULTS: Thirty-three subjects could be statistically analyzed using pooled meibum (13 with stable tear films (TBUTs > 10 s) and 20 with unstable tear films (TBUTs ≤ 10 s). Significant differences in meibum from subjects with unstable vs. stable TBUTs were found for the G1 fraction (medians 0.164 and 0.020, respectively; p = 0.012) and the G2 fraction (medians 0.244 and 0.272, respectively; p = 0.045). No differences were observed for the G3 and G4 fractions. Single orifice samples were not significantly different vs. pooled samples from the fellow eye, and eyelid sector samples (nasal, central and temporal) G2:G3 fractional components were not significantly different (p = 0.449). Spearman analysis suggested a significant inverse correlation between G1 fraction andTBUT (R = -0.351; p = 0.045). CONCLUSIONS: hsSRS microscopy allows compositional analysis of expressed meibum from humans which correlated to changes in TBUT. These findings support the hypothesis that hsSRS may be useful in classifying meibum quality and evaluating the effects of therapy.
Authors: Douglas Borchman; Gary N Foulks; Marta C Yappert; James Bell; Emily Wells; Shantanu Neravetla; Victoria Greenstone Journal: Invest Ophthalmol Vis Sci Date: 2011-06-01 Impact factor: 4.799
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Authors: Sun Woong Kim; Chang Rae Rho; Jinseor Kim; Yilu Xie; Richard C Prince; Khawla Mustafa; Eric O Potma; Donald J Brown; James V Jester Journal: Ocul Surf Date: 2020-04-30 Impact factor: 6.268