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Literature DB >> 3031670

In vivo aminoacylation of human and Xenopus suppressor tRNAs constructed by site-specific mutagenesis.

Abstract

Amber suppressor tRNA genes were constructed by site-specific mutagenesis of the anticodons of human lysine-inserting tRNA (tRNA(Lys)) and glutamine-inserting tRNA (tRNA(Gln)) genes, and a Xenopus laevis tyrosine-inserting tRNA (tRNA(Tyr)) gene. As previous in vitro studies in prokaryotes have shown that substitution of nucleotides in the anticodon region can profoundly affect tRNA aminoacylation, it is important to determine whether the mutation affects aminoacylation of these eukaryotic tRNAs. We present a method for quantitating the tRNA aminoacylation in vivo in mammalian cells, and we have determined that the suppressor tRNA(Tyr) is fully aminoacylated and suppressor tRNA(Lys) and tRNA(Gln) are aminoacylated 40-50% and 80%, respectively. This in vivo method of estimating aminoacylation may be applied to other mutations in the tRNA genes.

Entities: Chemical Gene Species

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Year: 1987 PMID： 3031670 PMCID： PMC304613 DOI： 10.1073/pnas.84.8.2185

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

33 in total

1. Nucleotide sequence studies of normal and genetically altered glycine transfer ribonucleic acids from Escherichia coli.

Authors: J W Roberts; J Carbon
Journal: J Biol Chem Date: 1975-07-25 Impact factor: 5.157

2. A single mutational modification of a tryptophan-specific transfer RNA permits aminoacylation by glutamine and translation of the codon UAG.

Authors: M Yaniv; W R Folk; P Berg; L Soll
Journal: J Mol Biol Date: 1974-06-25 Impact factor: 5.469

3. Molecular mechanism for missense suppression in E. coli.

Authors: J W Roberts; J Carbon
Journal: Nature Date: 1974-08-02 Impact factor: 49.962

4. Genetically and chemically derived missense suppressor transfer RNA's with altered enzymic aminoacylation rates.

Authors: J Carbon; J B Curry
Journal: J Mol Biol Date: 1968-12-14 Impact factor: 5.469

5. Glycine transfer RNA of Escherichia coli. II. Impaired GGA-recognition in strains containing a genetically altered transfer RNA; reversal by a secondary suppressor mutation.

Authors: J Carbon; C Squires; C W Hill
Journal: J Mol Biol Date: 1970-09-28 Impact factor: 5.469

In vivo aminoacylation of human and Xenopus suppressor tRNAs constructed by site-specific mutagenesis.

1. Nucleotide sequence studies of normal and genetically altered glycine transfer ribonucleic acids from Escherichia coli.

2. A single mutational modification of a tryptophan-specific transfer RNA permits aminoacylation by glutamine and translation of the codon UAG.

3. Molecular mechanism for missense suppression in E. coli.

4. Genetically and chemically derived missense suppressor transfer RNA's with altered enzymic aminoacylation rates.

5. Glycine transfer RNA of Escherichia coli. II. Impaired GGA-recognition in strains containing a genetically altered transfer RNA; reversal by a secondary suppressor mutation.

6. Suppressor su+7 inserts tryptophan in addition to glutamine.

Review 7. The role of the anticodon in recognition of tRNA by aminoacyl-tRNA synthetases.

8. A membrane-filter technique for the detection of complementary DNA.

9. beta 0 thalassemia, a nonsense mutation in man.

10. Three different missense suppressor mutations affecting the tRNA GGG Gly species of Escherichia coli.

1. Selection of retroviral reverse transcription primer is coordinated with tRNA biogenesis.

2. Analysis of aminoacyl- and peptidyl-tRNAs by gel electrophoresis.

3. Regulated expression of plant tRNA genes by the prokaryotic tet and lac repressors.

4. Enzymatic aminoacylation of sequence-specific RNA minihelices and hybrid duplexes with methionine.

5. Evidence for aminoacylation-induced conformational changes in human mitochondrial tRNAs.

6. Plant cytosolic tRNAHis possesses an exceptional C54 in the canonical TPsiC loop.

7. Initiator-elongator discrimination in vertebrate tRNAs for protein synthesis.

8. Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene.

9. Incorporation of excess wild-type and mutant tRNA(3Lys) into human immunodeficiency virus type 1.

10. Initiation of protein synthesis in mammalian cells with codons other than AUG and amino acids other than methionine.