Literature DB >> 30315459

13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells.

Marit van Gorsel1,2, Ilaria Elia1,2, Sarah-Maria Fendt3.   

Abstract

Metabolomics and 13C tracer analysis are state-of-the-art techniques that allow determining the concentration of metabolites and the activity of metabolic pathways, respectively. Three dimensional (3D) cultures of cancer cells constitute an enriched in vitro environment that can be used to assay anchorage-independent growth, spheroid formation, and extracellular matrix production by (cancer) cells. Here, we describe how to perform metabolomics and 13C tracer analysis in 3D cultures of cancer cells.

Entities:  

Keywords:  13C tracing; 3D cell culture; Cancer metabolism; GC-MS; LC-MS; Metabolomics; Spheroids

Mesh：

Substances：

Year:  2019        PMID： 30315459     DOI： 10.1007/978-1-4939-8769-6_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

1.  2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer.

Authors:  Arnaud Blomme; Catriona A Ford; Ernest Mui; Rachana Patel; Chara Ntala; Lauren E Jamieson; Mélanie Planque; Grace H McGregor; Paul Peixoto; Eric Hervouet; Colin Nixon; Mark Salji; Luke Gaughan; Elke Markert; Peter Repiscak; David Sumpton; Giovanny Rodriguez Blanco; Sergio Lilla; Jurre J Kamphorst; Duncan Graham; Karen Faulds; Gillian M MacKay; Sarah-Maria Fendt; Sara Zanivan; Hing Y Leung
Journal:  Nat Commun       Date:  2020-05-19       Impact factor: 14.919

2.  PHGDH heterogeneity potentiates cancer cell dissemination and metastasis.

Authors:  Matteo Rossi; Patricia Altea-Manzano; Margherita Demicco; Ginevra Doglioni; Laura Bornes; Marina Fukano; Anke Vandekeere; Alejandro M Cuadros; Juan Fernández-García; Carla Riera-Domingo; Cristina Jauset; Mélanie Planque; H Furkan Alkan; David Nittner; Dongmei Zuo; Lindsay A Broadfield; Sweta Parik; Antonino Alejandro Pane; Francesca Rizzollo; Gianmarco Rinaldi; Tao Zhang; Shao Thing Teoh; Arin B Aurora; Panagiotis Karras; Ines Vermeire; Dorien Broekaert; Joke Van Elsen; Maximilian M L Knott; Martin F Orth; Sofie Demeyer; Guy Eelen; Lacey E Dobrolecki; Ayse Bassez; Thomas Van Brussel; Karl Sotlar; Michael T Lewis; Harald Bartsch; Manfred Wuhrer; Peter van Veelen; Peter Carmeliet; Jan Cools; Sean J Morrison; Jean-Christophe Marine; Diether Lambrechts; Massimiliano Mazzone; Gregory J Hannon; Sophia Y Lunt; Thomas G P Grünewald; Morag Park; Jacco van Rheenen; Sarah-Maria Fendt
Journal:  Nature       Date:  2022-05-18       Impact factor: 69.504

3.  In Vivo Evidence for Serine Biosynthesis-Defined Sensitivity of Lung Metastasis, but Not of Primary Breast Tumors, to mTORC1 Inhibition.

Authors:  Gianmarco Rinaldi; Erica Pranzini; Joke Van Elsen; Dorien Broekaert; Cornelius M Funk; Mélanie Planque; Ginevra Doglioni; Patricia Altea-Manzano; Matteo Rossi; Vincent Geldhof; Shao Thing Teoh; Christina Ross; Kent W Hunter; Sophia Y Lunt; Thomas G P Grünewald; Sarah-Maria Fendt
Journal:  Mol Cell       Date:  2020-12-18       Impact factor: 19.328

4.  Effects of the Novel PFKFB3 Inhibitor KAN0438757 on Colorectal Cancer Cells and Its Systemic Toxicity Evaluation In Vivo.

Authors:  Tiago De Oliveira; Tina Goldhardt; Marcus Edelmann; Torben Rogge; Karsten Rauch; Nikola Dobrinov Kyuchukov; Kerstin Menck; Annalen Bleckman; Joanna Kalucka; Shawez Khan; Jochen Gaedcke; Martin Haubrock; Tim Beissbarth; Hanibal Bohnenberger; Mélanie Planque; Sarah-Maria Fendt; Lutz Ackermann; Michael Ghadimi; Lena-Christin Conradi
Journal:  Cancers (Basel)       Date:  2021-02-28       Impact factor: 6.639

5.  Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasis.

Authors:  Madeleine Dorsch; Manuela Kowalczyk; Mélanie Planque; Geronimo Heilmann; Sebastian Urban; Philip Dujardin; Jan Forster; Kristina Ueffing; Silke Nothdurft; Sebastian Oeck; Annika Paul; Sven T Liffers; Farnusch Kaschani; Markus Kaiser; Alexander Schramm; Jens T Siveke; Monte M Winslow; Sarah-Maria Fendt; Perihan Nalbant; Barbara M Grüner
Journal:  Cell Rep       Date:  2021-11-23       Impact factor: 9.423

Review 6.  Resolving Metabolic Heterogeneity in Experimental Models of the Tumor Microenvironment from a Stable Isotope Resolved Metabolomics Perspective.

Authors:  Teresa W-M Fan; Richard M Higashi; Yelena Chernayavskaya; Andrew N Lane
Journal:  Metabolites       Date:  2020-06-15

7.  In crystallo screening for proline analog inhibitors of the proline cycle enzyme PYCR1.

Authors:  Emily M Christensen; Alexandra N Bogner; Anke Vandekeere; Gabriela S Tam; Sagar M Patel; Donald F Becker; Sarah-Maria Fendt; John J Tanner
Journal:  J Biol Chem       Date:  2020-10-27       Impact factor: 5.157

8.  SOX9-induced Generation of Functional Astrocytes Supporting Neuronal Maturation in an All-human System.

Authors:  Katrien Neyrinck; Johanna Van Den Daele; Tim Vervliet; Jonathan De Smedt; Keimpe Wierda; Melissa Nijs; Tom Vanbokhoven; Astrid D'hondt; Mélanie Planque; Sarah-Maria Fendt; Pei-Yu Shih; Frederik Seibt; Juan Pita Almenar; Mohamed Kreir; Devesh Kumar; Vania Broccoli; Geert Bultynck; Andreas Ebneth; Alfredo Cabrera-Socorro; Catherine Verfaillie
Journal:  Stem Cell Rev Rep       Date:  2021-05-12       Impact factor: 5.739
  8 in total

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