Simon Fishel1, Alison Campbell2, Sue Montgomery3, Rachel Smith4, Lynne Nice5, Samantha Duffy3, Lucy Jenner6, Kathryn Berrisford6, Louise Kellam6, Rob Smith7, Fiona Foad8, Ashley Beccles1. 1. CARE Fertility GroupJohn Webster House6 Lawrence DriveNottingham Business ParkNottinghamNG8 6PZUK. 2. CARE Fertility GroupJohn Webster House6 Lawrence DriveNottingham Business ParkNottinghamNG8 6PZUK. Electronic address: alison.campbell@carefertility.com. 3. CARE ManchesterVictoria Park108-112 Daisy Bank RoadManchesterM14 5QHUK. 4. CARE Sheffield24-26 Glen RoadSheffieldS7 1RAUK. 5. CARE NorthamptonCliftonville67 The AvenueNorthamptonNN1 5BTUK. 6. CARE NottinghamJohn Webster HouseNottingham Business Park6 Lawrence DriveNottinghamNG8 6PZUK. 7. CARE LondonPark Lorne111 Park RoadLondonNW8 7JLUK. 8. CARE DublinBeacon CARE FertilityBeacon CourtSandyfordDublin 18Ireland.
Abstract
RESEARCH QUESTION: Can blastocysts leading to live births be ranked according to morphokinetic-based algorithms? DESIGN: Retrospective analysis of 781 single blastocyst embryo transfers, including all patient clinical factors that might be potential confounders for the primary outcome measure of live birth, was weighed using separate multi-variable logistic regression models. RESULTS: There was strong evidence of effect of embryo rank on odds of live birth. Embryos were classified A, B, C or D according to calculated variables; time to start (tSB) and duration (dB{tB - tSB}) of blastulation. Embryos of rank D were less likely to result in live birth than embryos of rank A (odds ratio [OR] 0.3046; 95% confidence interval [CI] 0.129, 0.660; P < 0.005). Embryos ranked B were less likely to result in live birth than those ranked A (OR 0.7114; 95% Cl 0.505, 1.001; P < 0.01), and embryos ranked C were less likely to result in live birth than those ranked A (OR 0.6501, 95% Cl 0.373, 1.118; P < 0.01). Overall, the LRT (Likelihood Ratio Test) p-value for embryo rank shows that there is strong evidence that embryo rank is informative as a whole in discriminating between live birth and no live birth outcomes (p = 0.0101). The incidence of live birth was 52.5% from rank A, 39.2% from rank B, 31.4% from rank C and 13.2% from rank D. CONCLUSIONS: Time-lapse imaging morphokinetic-based algorithms for blastocysts can provide objective hierarchical ranking of embryos for predicting live birth and may have greater discriminating power than conventional blastocyst morphology assessment.
RESEARCH QUESTION: Can blastocysts leading to live births be ranked according to morphokinetic-based algorithms? DESIGN: Retrospective analysis of 781 single blastocyst embryo transfers, including all patient clinical factors that might be potential confounders for the primary outcome measure of live birth, was weighed using separate multi-variable logistic regression models. RESULTS: There was strong evidence of effect of embryo rank on odds of live birth. Embryos were classified A, B, C or D according to calculated variables; time to start (tSB) and duration (dB{tB - tSB}) of blastulation. Embryos of rank D were less likely to result in live birth than embryos of rank A (odds ratio [OR] 0.3046; 95% confidence interval [CI] 0.129, 0.660; P < 0.005). Embryos ranked B were less likely to result in live birth than those ranked A (OR 0.7114; 95% Cl 0.505, 1.001; P < 0.01), and embryos ranked C were less likely to result in live birth than those ranked A (OR 0.6501, 95% Cl 0.373, 1.118; P < 0.01). Overall, the LRT (Likelihood Ratio Test) p-value for embryo rank shows that there is strong evidence that embryo rank is informative as a whole in discriminating between live birth and no live birth outcomes (p = 0.0101). The incidence of live birth was 52.5% from rank A, 39.2% from rank B, 31.4% from rank C and 13.2% from rank D. CONCLUSIONS: Time-lapse imaging morphokinetic-based algorithms for blastocysts can provide objective hierarchical ranking of embryos for predicting live birth and may have greater discriminating power than conventional blastocyst morphology assessment.
Authors: Susanna Apter; Thomas Ebner; Thomas Freour; Yves Guns; Borut Kovacic; Nathalie Le Clef; Monica Marques; Marcos Meseguer; Debbie Montjean; Ioannis Sfontouris; Roger Sturmey; Giovanni Coticchio Journal: Hum Reprod Open Date: 2020-03-19
Authors: Katarzyna Olszak-Wąsik; Anna Bednarska-Czerwińska; Anita Olejek; Andrzej Tukiendorf Journal: Oxid Med Cell Longev Date: 2019-11-26 Impact factor: 6.543