Yu-Chi Lin1, Makoto Kuroda2, Satowa Suzuki3, Jung-Jung Mu4. 1. Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, 161 Kun-Yang St., Taipei 11561, Taiwan. 2. Pathogen Genomics Center, National Institute of Infectious Diseases, Japan. 3. Antimicrobial Research Center, National Institute of Infectious Diseases, Japan. 4. Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, 161 Kun-Yang St., Taipei 11561, Taiwan. Electronic address: jjmu@cdc.gov.tw.
Abstract
OBJECTIVES: Multidrug-resistant bacteria have become a serious threat worldwide. In particular, the coexistence of carbapenemase genes and mcr-1 leaves few available treatment options. Here we report a multidrug-resistant Escherichia coli isolate harbouring both mcr-1 and blaNDM-9 from a patient with a urinary tract infection. METHODS: Antimicrobial susceptibility and resistance genes of the E. coli isolate were characterised. Furthermore, the assembled genome sequences of mcr-1- and blaNDM-9-carrying plasmids were determined and comparative genetic analysis with closely related plasmids was carried out. RESULTS: Three contigs were assembled comprising the E. coli chromosome and two plasmids harbouring mcr-1 (p5CRE51-MCR-1) and blaNDM-9 (p5CRE51-NDM-9), respectively. Whole-genome sequencing revealed that the two antimicrobial resistance genes are located on individual plasmids. CONCLUSIONS: The emergence of coexistence of carbapenemase genes and mcr-1 in Enterobacteriaceae highlights a serious threat to antimicrobial therapy.
OBJECTIVES: Multidrug-resistant bacteria have become a serious threat worldwide. In particular, the coexistence of carbapenemase genes and mcr-1 leaves few available treatment options. Here we report a multidrug-resistant Escherichia coli isolate harbouring both mcr-1 and blaNDM-9 from a patient with a urinary tract infection. METHODS: Antimicrobial susceptibility and resistance genes of the E. coli isolate were characterised. Furthermore, the assembled genome sequences of mcr-1- and blaNDM-9-carrying plasmids were determined and comparative genetic analysis with closely related plasmids was carried out. RESULTS: Three contigs were assembled comprising the E. coli chromosome and two plasmids harbouring mcr-1 (p5CRE51-MCR-1) and blaNDM-9 (p5CRE51-NDM-9), respectively. Whole-genome sequencing revealed that the two antimicrobial resistance genes are located on individual plasmids. CONCLUSIONS: The emergence of coexistence of carbapenemase genes and mcr-1 in Enterobacteriaceae highlights a serious threat to antimicrobial therapy.
Authors: Diego Faccone; Melina Rapoport; Ezequiel Albornoz; Federico Celaya; Juan De Mendieta; Denise De Belder; Celeste Lucero; Sonia Gomez; Diego Danze; Fernando Pasteran; Alejandra Corso Journal: Rev Panam Salud Publica Date: 2020-09-23
Authors: Le Phuong Nguyen; Chul Soon Park; Naina Adren Pinto; Hyunsook Lee; Hyun Soo Seo; Thao Nguyen Vu; Hung Mai; An H T Pham; Eris Jang; Young Lag Cho; Karrie Goglin; Kevin Nguyen; Richard White; Roshan D'Souza; Derrick E Fouts; Dongeun Yong Journal: Pharmaceuticals (Basel) Date: 2021-04-16