Literature DB >> 3031023

IS2 activates the ilvA gene of Pseudomonas cepacia in Escherichia coli.

G Barsomian, T G Lessie.   

Abstract

The ilvA gene of Pseudomonas cepacia was expressed poorly in Escherichia coli. Insertion of IS2 upstream of the cloned gene dramatically increased its transcription, resulting in an 85-fold increase in threonine dehydratase (deaminase) specific activity. The results confirm earlier reports that IS2 promotes efficient expression of foreign genes in E. coli.

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Year:  1987        PMID： 3031023      PMCID： PMC212018          DOI： 10.1128/jb.169.4.1777-1779.1987

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  16 in total

1.  Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors:  A C Chang; S N Cohen
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

2.  Gel electrophoresis of restriction fragments.

Authors:  E Southern
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

3.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

4.  Nucleotide sequence of the transposable DNA-element IS2.

Authors:  D Ghosal; H Sommer; H Saedler
Journal:  Nucleic Acids Res       Date:  1979-03       Impact factor: 16.971

5.  The analysis of nucleic acids in gels using glyoxal and acridine orange.

Authors:  G G Carmichael; G K McMaster
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Expression of the Aspergillus nidulans argB gene in Escherichia coli.

Authors:  A Dmochowska; B Berse; I Kowalska; M Skrzypek; P Wegleński
Journal:  J Gen Microbiol       Date:  1986-06

7.  Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

Authors:  J A Meyers; D Sanchez; L P Elwell; S Falkow
Journal:  J Bacteriol       Date:  1976-09       Impact factor: 3.490

8.  Physical organization of the ilvEDAC genes of Escherichia coli strain K-12.

Authors:  G M McCorkle; T D Leathers; H E Umbarger
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

9.  Control of expression of a cloned yeast (Saccharomyces cerevisiae) gene (trp5) by a bacterial insertion element (IS2).

Authors:  A Walz; B Ratzkin; J Carbon
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

10.  Properties of threonine deaminase from a bacterium able to use threonine as sole source of carbon.

Authors:  T G Lessie; H R Whiteley
Journal:  J Bacteriol       Date:  1969-11       Impact factor: 3.490
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  6 in total

1.  Genes for 2,4,5-trichlorophenoxyacetic acid metabolism in Burkholderia cepacia AC1100: characterization of the tftC and tftD genes and locations of the tft operons on multiple replicons.

Authors:  A Hübner; C E Danganan; L Xun; A M Chakrabarty; W Hendrickson
Journal:  Appl Environ Microbiol       Date:  1998-06       Impact factor: 4.792

2.  Characterization of a sequence (hlyR) which enhances synthesis and secretion of hemolysin in Escherichia coli.

Authors:  M Vogel; J Hess; I Then; A Juarez; W Goebel
Journal:  Mol Gen Genet       Date:  1988-04

3.  Transposition of IS2 into the hemB gene of Escherichia coli K-12.

Authors:  L A Lewis; D Lewis; V Persaud; S Gopaul; B Turner
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

4.  Activation of the lac genes of Tn951 by insertion sequences from Pseudomonas cepacia.

Authors:  M S Wood; C Lory; T G Lessie
Journal:  J Bacteriol       Date:  1990-04       Impact factor: 3.490

5.  Marked phenotypic variability in Pseudomonas cepacia isolated from a patient with cystic fibrosis.

Authors:  G Y Larsen; T L Stull; J L Burns
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

6.  Multiple replicons constituting the genome of Pseudomonas cepacia 17616.

Authors:  H P Cheng; T G Lessie
Journal:  J Bacteriol       Date:  1994-07       Impact factor: 3.490
  6 in total

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