Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Sensitive, rapid, and specific bioassay for the determination of antilipogenic compounds.

Literature DB >> 303076

Sensitive, rapid, and specific bioassay for the determination of antilipogenic compounds.

Abstract

A sensitive and rapid bioassay for the determination of the antilipogenic compounds cerulenin and CM-55 is described. The bioassay is based on the inhibitory effect of cerulenin and CM-55 on the in vivo luminescence of an aldehyde-requiring mutant of the marine bacterium Beneckea harveyi. A total quantity as low as 0.1 mug of cerulenin can be determined within 15 min with an error of +/-2%. The bioassay, as presented, is specific for compounds that are known to inhibit fatty acid biosynthesis and, as such, it might be used as a general screening method for the detection of antilipogenic compounds.

Entities: Chemical Species

Mesh：

Substances：

Year: 1977 PMID： 303076 PMCID： PMC429910 DOI： 10.1128/AAC.12.3.308

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

9 in total

1. Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast.

Authors: T Ono; T Kesado; J Awaya; S Omura
Journal: Biochem Biophys Res Commun Date: 1974-04-23 Impact factor: 3.575

2. The action mechanism of cerulenin. I. Effect of cerulenin on sterol and fatty acid biosynthesis in yeast.

Authors: S Nomura; T Horiuchi; S Omura; T Hata
Journal: J Biochem Date: 1972-05 Impact factor: 3.387

3. Inhibition of fatty acid synthesis by the antibiotic cerulenin. Specific inactivation of beta-ketoacyl-acyl carrier protein synthetase.

Authors: G D'Agnolo; I S Rosenfeld; J Awaya; S Omura; P R Vagelos
Journal: Biochim Biophys Acta Date: 1973-11-29

4. Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometer.

Authors: P E Stanley
Journal: Anal Biochem Date: 1971-02 Impact factor: 3.365

4 in total

1. Growth, luminescence, respiration, and the ATP pool during autoinduction in Beneckea harveyi.

Authors: S Ulitzur; J W Hastings
Journal: J Bacteriol Date: 1978-03 Impact factor: 3.490

2. Control of aldehyde synthesis in the luminous bacterium Beneckea harveyi.

Authors: S Ulitzur; J W Hastings
Journal: J Bacteriol Date: 1979-02 Impact factor: 3.490

3. Inhibition of Vibrio harveyi bioluminescence by cerulenin: in vivo evidence for covalent modification of the reductase enzyme involved in aldehyde synthesis.

Authors: D M Byers; E A Meighen
Journal: J Bacteriol Date: 1989-07 Impact factor: 3.490

4. Elongation of exogenous fatty acids by the bioluminescent bacterium Vibrio harveyi.

Authors: D M Byers
Journal: J Bacteriol Date: 1989-01 Impact factor: 3.490

4 in total

Sensitive, rapid, and specific bioassay for the determination of antilipogenic compounds.

1. Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast.

2. The action mechanism of cerulenin. I. Effect of cerulenin on sterol and fatty acid biosynthesis in yeast.

3. Inhibition of fatty acid synthesis by the antibiotic cerulenin. Specific inactivation of beta-ketoacyl-acyl carrier protein synthetase.

4. Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometer.

5. Revised structure of cerulenin.

6. The inhibition of bacterial luciferase by mixed function oxidase inhibitors.

Review 7. The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis.

8. Inhibition of fatty acid synthetases by the antibiotic cerulenin.

9. Mechanism of action of CM-55, a synthetic analogue of the antilipogenic antibiotic cerulenin.

1. Growth, luminescence, respiration, and the ATP pool during autoinduction in Beneckea harveyi.

2. Control of aldehyde synthesis in the luminous bacterium Beneckea harveyi.

3. Inhibition of Vibrio harveyi bioluminescence by cerulenin: in vivo evidence for covalent modification of the reductase enzyme involved in aldehyde synthesis.

4. Elongation of exogenous fatty acids by the bioluminescent bacterium Vibrio harveyi.