Yong Kwan Lim1,2, Ae Ja Park2, Oh Joo Kweon2,3, Jee-Hye Choi2. 1. Department of Laboratory Medicine, Armed Forces Capital Hospital, Gyeonggi-do, South Korea. 2. Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, South Korea. 3. Department of Laboratory Medicine, Aerospace Medical Center, Chungcheongbuk-do, South Korea.
Abstract
Objectives: We evaluated performance and measurement uncertainty of the newly released ARCHITECT 25-OH vitamin D 5P02 assay (Abbott Diagnostics, Abbott Park, IL). Methods: In total, 300 samples were used to compare the results from ELECSYS Vitamin D Total (Roche Diagnostics, Mannheim, Germany) and ADVIA Centaur Vitamin D Total (Siemens, Tarrytown, NY). To quantify the measurement uncertainty, 25 samples and four levels of standard reference material (SRM) were measured using isotope dilution-liquid chromatography-tandem mass spectrometry according to international guidelines. Results: The results of the ARCHITECT assay were equivalent to other immunoassays, but correlation coefficients were lower than the recommended criterion. SRM level 2 was considered adequate for uncertainty estimation, and the expanded measurement uncertainty of the ARCHITECT assay was 4.2%, which was superior to the other two assays. Conclusions: The restandardized ARCHITECT assay has acceptable performance in a clinical setting. However, there is still a need for further standardization of total vitamin D measurement among the automated immunoassays.
Objectives: We evaluated performance and measurement uncertainty of the newly released ARCHITECT 25-OH vitamin D 5P02 assay (Abbott Diagnostics, Abbott Park, IL). Methods: In total, 300 samples were used to compare the results from ELECSYS Vitamin D Total (Roche Diagnostics, Mannheim, Germany) and ADVIA Centaur Vitamin D Total (Siemens, Tarrytown, NY). To quantify the measurement uncertainty, 25 samples and four levels of standard reference material (SRM) were measured using isotope dilution-liquid chromatography-tandem mass spectrometry according to international guidelines. Results: The results of the ARCHITECT assay were equivalent to other immunoassays, but correlation coefficients were lower than the recommended criterion. SRM level 2 was considered adequate for uncertainty estimation, and the expanded measurement uncertainty of the ARCHITECT assay was 4.2%, which was superior to the other two assays. Conclusions: The restandardized ARCHITECT assay has acceptable performance in a clinical setting. However, there is still a need for further standardization of total vitamin D measurement among the automated immunoassays.
Authors: Stephen A Wise; Johanna E Camara; Carolyn Q Burdette; Grace Hahm; Federica Nalin; Adam J Kuszak; Joyce Merkel; Ramón A Durazo-Arvizu; Emma L Williams; Andrew N Hoofnagle; Fiona Ivison; Ralf Fischer; Jody M W van den Ouweland; Chung S Ho; Emmett W K Law; Jean-Nicolas Simard; Renaud Gonthier; Brett Holmquist; Sarah Meadows; Lorna Cox; Kimberly Robyak; Michael H Creer; Robert Fitzgerald; Michael W Clarke; Norma Breen; Pierre Lukas; Étienne Cavalier; Christopher T Sempos Journal: Anal Bioanal Chem Date: 2021-08-25 Impact factor: 4.142
Authors: Stephen A Wise; Johanna E Camara; Carolyn Q Burdette; Grace Hahm; Federica Nalin; Adam J Kuszak; Joyce Merkel; Ramón A Durazo-Arvizu; Emma L Williams; Christian Popp; Christian Beckert; Jan Schultess; Glen Van Slooten; Carole Tourneur; Camille Pease; Ravi Kaul; Alfredo Villarreal; Marcelo Cidade Batista; Heather Pham; Alex Bennett; Eugene Jansen; Dilshad Ahmed Khan; Mark Kilbane; Patrick J Twomey; James Freeman; Neil Parker; Sohail Mushtaq; Christine Simpson; Pierre Lukas; Étienne Cavalier; Christopher T Sempos Journal: Anal Bioanal Chem Date: 2021-08-25 Impact factor: 4.142
Authors: Barbara Altieri; Etienne Cavalier; Harjit Pal Bhattoa; Faustino R Pérez-López; María T López-Baena; Gonzalo R Pérez-Roncero; Peter Chedraui; Cedric Annweiler; Silvia Della Casa; Sieglinde Zelzer; Markus Herrmann; Antongiulio Faggiano; Annamaria Colao; Michael F Holick Journal: Eur J Clin Nutr Date: 2020-01-06 Impact factor: 4.016