Literature DB >> 3030293

Identification of the Rts 1 DNA fragment responsible for temperature sensitive growth of host cells harboring a drug resistant factor Rts 1.

N Okawa, M Tanaka, S Finver, A Kaji.   

Abstract

We have placed a kanamycin resistance SalI fragment (3.65 Kb) from the drug resistance factor Rts1 into pUC19 and pBR322. These chimeric plasmids containing the kanamycin resistance fragment from Rts1 cause temperature sensitive growth in E coli. The orientation of the kanamycin resistance fragment in the vector plasmids does not influence this phenotype.

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Year:  1987        PMID： 3030293     DOI： 10.1016/0006-291x(87)91526-9

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  5 in total

1.  Three short fragments of Rts1 DNA are responsible for the temperature-sensitive growth phenotype (Tsg) of host bacteria.

Authors:  S Mochida; H Tsuchiya; K Mori; A Kaji
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490

2.  Effects of mutations in the repA gene of plasmid Rts1 on plasmid replication and autorepressor function.

Authors:  Y Terawaki; H Nozue; H Zeng; T Hayashi; Y Kamio; Y Itoh
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

3.  Site-directed mutations in the repA C-terminal region of plasmid Rts1: pleiotropic effects on the replication and autorepressor functions.

Authors:  H Zeng; T Hayashi; Y Terawaki
Journal:  J Bacteriol       Date:  1990-05       Impact factor: 3.490

4.  Complete nucleotide sequence of plasmid Rts1: implications for evolution of large plasmid genomes.

Authors:  Takahiro Murata; Makoto Ohnishi; Takeshi Ara; Jun Kaneko; Chang-Gyun Han; Yong Fang Li; Kayoko Takashima; Hideaki Nojima; Keisuke Nakayama; Akira Kaji; Yoshiyuki Kamio; Takeyoshi Miki; Hirotada Mori; Eiichi Ohtsubo; Yoshiro Terawaki; Tetsuya Hayashi
Journal:  J Bacteriol       Date:  2002-06       Impact factor: 3.490

5.  Nucleotide sequence of an Rts1 fragment causing temperature-dependent instability.

Authors:  M Tanaka; N Okawa; K Mori; Y Suyama; A Kaji
Journal:  J Bacteriol       Date:  1988-03       Impact factor: 3.490
  5 in total

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