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Literature DB >> 30297356

The Expanding Molecular Genetics Tool Kit in Chlamydia.

Raphael H Valdivia¹, Robert J Bastidas².

Abstract

Chlamydia has emerged as an important model system for the study of host pathogen interactions, in part due to a resurgence in the development of tools for its molecular genetic manipulation. An additional tool, published by Keb et al. (G. Keb, R. Hayman, and K. A. Fields, J. Bacteriol. 200:e00479-18, 2018, https://doi.org/10.1128/JB.00479-18), now allows for custom genetic engineering of genomic regions that were traditionally recalcitrant to genetic manipulation, such as genes within operons. This new method will be an essential instrument for the elucidation of Chlamydia-host interactions.

Entities: Disease Gene

Keywords: Chlamydia genetics; Chlamydia trachomatiszzm321990

Mesh：

Year: 2018 PMID： 30297356 PMCID： PMC6256018 DOI： 10.1128/JB.00590-18

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

19 in total

1. Chlamydia trachomatis plasmid-encoded Pgp4 is a transcriptional regulator of virulence-associated genes.

Authors: Lihua Song; John H Carlson; William M Whitmire; Laszlo Kari; Kimmo Virtaneva; Daniel E Sturdevant; Heather Watkins; Bing Zhou; Gail L Sturdevant; Stephen F Porcella; Grant McClarty; Harlan D Caldwell
Journal: Infect Immun Date: 2013-01-14 Impact factor: 3.441

2. Integrating chemical mutagenesis and whole-genome sequencing as a platform for forward and reverse genetic analysis of Chlamydia.

Authors: Marcela Kokes; Joe Dan Dunn; Joshua A Granek; Bidong D Nguyen; Jeffrey R Barker; Raphael H Valdivia; Robert J Bastidas
Journal: Cell Host Microbe Date: 2015-04-23 Impact factor: 21.023

3. Polymorphisms in the nine polymorphic membrane proteins of Chlamydia trachomatis across all serovars: evidence for serovar Da recombination and correlation with tissue tropism.

Authors: João P Gomes; Alexandra Nunes; William J Bruno; Maria J Borrego; Carlos Florindo; Deborah Dean
Journal: J Bacteriol Date: 2006-01 Impact factor: 3.490

4. Lateral gene transfer in vitro in the intracellular pathogen Chlamydia trachomatis.

Authors: Robert Demars; Jason Weinfurter; Erin Guex; John Lin; Yvonne Potucek
Journal: J Bacteriol Date: 2006-11-22 Impact factor: 3.490

5. Fluorescence-Reported Allelic Exchange Mutagenesis Reveals a Role for Chlamydia trachomatis TmeA in Invasion That Is Independent of Host AHNAK.

Authors: M J McKuen; K E Mueller; Y S Bae; K A Fields
Journal: Infect Immun Date: 2017-11-17 Impact factor: 3.441

6. Expression of recombinant DNA introduced into Chlamydia trachomatis by electroporation.

Authors: J E Tam; C H Davis; P B Wyrick
Journal: Can J Microbiol Date: 1994-07 Impact factor: 2.419

7. Floxed-Cassette Allelic Exchange Mutagenesis Enables Markerless Gene Deletion in Chlamydia trachomatis and Can Reverse Cassette-Induced Polar Effects.

Authors: G Keb; R Hayman; K A Fields
Journal: J Bacteriol Date: 2018-11-26 Impact factor: 3.490

8. Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector.

Authors: Yibing Wang; Simona Kahane; Lesley T Cutcliffe; Rachel J Skilton; Paul R Lambden; Ian N Clarke
Journal: PLoS Pathog Date: 2011-09-22 Impact factor: 6.823

9. Gene Deletion by Fluorescence-Reported Allelic Exchange Mutagenesis in Chlamydia trachomatis.

Authors: Konrad E Mueller; Katerina Wolf; Kenneth A Fields
Journal: MBio Date: 2016-01-19 Impact factor: 7.867

10. Application of β-lactamase reporter fusions as an indicator of effector protein secretion during infections with the obligate intracellular pathogen Chlamydia trachomatis.

Authors: Konrad E Mueller; Kenneth A Fields
Journal: PLoS One Date: 2015-08-10 Impact factor: 3.240

4 in total