Literature DB >> 3029711

DNA orientation using specific avidin-ferritin biotin end labelling.

B Theveny, B Revet.   

Abstract

The orientation of DNA molecules has been determined by labelling one of the molecule end with a Biotin-labelled analog of dTTP (Bio-dUTP) and then by complexing the Bio-dUTP with Avidin-Ferritin. DNAs of phi X174, pBR322 and SV40 were end labelled with Bio-dUTP and imaged by Electron Microscopy (EM). This is a rapid, general method to unambiguously determine the orientation of DNA molecules for precise mapping and quantification of DNA secondary structures or protein-DNA interaction sites using EM.

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Year:  1987        PMID： 3029711      PMCID： PMC340500          DOI： 10.1093/nar/15.3.947

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

2.  Cloning of human mitochondrial DNA in Escherichia coli.

Authors:  J Drouin
Journal:  J Mol Biol       Date:  1980-06-15       Impact factor: 5.469

3.  Immunological method for mapping genes on Drosophila polytene chromosomes.

Authors:  P R Langer-Safer; M Levine; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

4.  Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.

Authors:  R Roychoudhury; E Jay; R Wu
Journal:  Nucleic Acids Res       Date:  1976-01       Impact factor: 16.971

5.  Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

Authors:  J J Leary; D J Brigati; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

6.  Biotin-labeled oligonucleotides: enzymatic synthesis and use as hybridization probes.

Authors:  A Murasugi; R B Wallace
Journal:  DNA       Date:  1984-06

7.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors:  P R Langer; A A Waldrop; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

8.  Three dimensional association of double-stranded helices are produced in conditions for Z-DNA formation.

Authors:  B Revet; E Delain; R Dante; A Niveleau
Journal:  J Biomol Struct Dyn       Date:  1983-12

9.  Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.

Authors:  D J Brigati; D Myerson; J J Leary; B Spalholz; S Z Travis; C K Fong; G D Hsiung; D C Ward
Journal:  Virology       Date:  1983-04-15       Impact factor: 3.616

10.  Torsional stress induces left-handed helical stretches in DNA of natural base sequence: circular dichroism and antibody binding.

Authors:  E Di Capua; A Stasiak; T Koller; S Brahms; R Thomae; F M Pohl
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  4 in total

1.  Mapping the intrinsic curvature and flexibility along the DNA chain.

Authors:  G Zuccheri; A Scipioni; V Cavaliere; G Gargiulo; P De Santis; B Samorì
Journal:  Proc Natl Acad Sci U S A       Date:  2001-02-27       Impact factor: 11.205

2.  Binding of the transcription activator NRI (NTRC) to a supercoiled DNA segment imitates association with the natural enhancer: an electron microscopic investigation.

Authors:  B Révet; S Brahms; G Brahms
Journal:  Proc Natl Acad Sci U S A       Date:  1995-08-01       Impact factor: 11.205

3.  Short unligated sticky ends enable the observation of circularised DNA by atomic force and electron microscopies.

Authors:  B Révet; A Fourcade
Journal:  Nucleic Acids Res       Date:  1998-05-01       Impact factor: 16.971

4.  Electron microscopy mapping of pBR322 DNA curvature. Comparison with theoretical models.

Authors:  G Muzard; B Théveny; B Révet
Journal:  EMBO J       Date:  1990-04       Impact factor: 11.598
  4 in total

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