Jieli Li1, Elizabeth A Wagar1, Qing H Meng2. 1. Department of Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. 2. Department of Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. Electronic address: qhmeng@mdanderson.org.
Abstract
BACKGROUND: Biotinylated antibodies and analogues are currently used in many immunoassays while biotin is widely used as a dietary supplement. Thus, biotin interference is an emerging issue for clinical laboratories. METHODS: Various concentrations of biotin solutions were prepared using pooled patient serum samples. All analytes were measured by sandwich or competitive immunoassay on the Roche Cobas 8000 e602 platform. RESULTS: Some of the sandwich immunoassay results were falsely decreased to different extents by different biotin levels, while some of the competitive immunoassay results were falsely increased. The most notable false reductions were in high-sensitivity troponin T, thyroid-stimulating hormone, and follicle-stimulating hormone results, while the most notable false increases were in triiodothyronine and vitamin D results. Other immunoassay results were also affected to some extent by biotin interference. CONCLUSIONS: Biotin can interfere in immunoassays and result in aberrant test results. Clinicians should use caution in interpreting abnormal results in patients who ingest biotin.
BACKGROUND: Biotinylated antibodies and analogues are currently used in many immunoassays while biotin is widely used as a dietary supplement. Thus, biotin interference is an emerging issue for clinical laboratories. METHODS: Various concentrations of biotin solutions were prepared using pooled patient serum samples. All analytes were measured by sandwich or competitive immunoassay on the Roche Cobas 8000 e602 platform. RESULTS: Some of the sandwich immunoassay results were falsely decreased to different extents by different biotin levels, while some of the competitive immunoassay results were falsely increased. The most notable false reductions were in high-sensitivity troponin T, thyroid-stimulating hormone, and follicle-stimulating hormone results, while the most notable false increases were in triiodothyronine and vitamin D results. Other immunoassay results were also affected to some extent by biotin interference. CONCLUSIONS:Biotin can interfere in immunoassays and result in aberrant test results. Clinicians should use caution in interpreting abnormal results in patients who ingest biotin.
Authors: Darcy Adin; Lisa Freeman; Rebecca Stepien; John E Rush; Sonja Tjostheim; Heidi Kellihan; Michael Aherne; Michelle Vereb; Robert Goldberg Journal: J Vet Intern Med Date: 2021-02-27 Impact factor: 3.333
Authors: Jose C Alvarez-Payares; Jesus David Bello-Simanca; Edwin De Jesus De La Peña-Arrieta; Jose Emilio Agamez-Gomez; Jhon Edwar Garcia-Rueda; Amilkar Rodriguez-Arrieta; Luis Antonio Rodriguez-Arrieta Journal: Front Endocrinol (Lausanne) Date: 2021-09-07 Impact factor: 5.555