Mark E Obrenovich1,2,3,4, Curtis J Donskey5,6, Isaac T Schiefer4, Rodolfo Bongiovanni2, Ling Li7, George E Jaskiw8,9. 1. Pathology & Laboratory Medicine Service, Louis Stokes Cleveland Department of Veteran's Affairs Medical Center, Cleveland, OH, 44106, USA. 2. Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, 44106, USA. 3. Department of Chemistry, Case Western Reserve University, Cleveland, OH, 44106, USA. 4. Department of Medicinal & Biological Chemistry, College of Pharmacy & Pharmaceutical Sciences, University of Toledo, Toledo, OH, 43614, USA. 5. Infectious Disease Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, 44106, USA. 6. School of Medicine, Case Western Reserve University, Cleveland, OH, 44106, USA. 7. Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, 44106, USA. 8. Psychiatry Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, 44106, USA. 9. Department of Psychiatry, Case Western Reserve University, Cleveland, OH, 44106, USA.
Abstract
AIM: Co-metabolism between a human host and the gastrointestinal microbiota generates many small phenolic molecules such as 3-hydroxy-3-(3-hydroxyphenyl)propanoic acid (3,3-HPHPA), which are reported to be elevated in schizophrenia and autism. Characterization of these chemicals, however, has been limited by analytic challenges. METHODOLOGY/ RESULTS: We applied HPLC to separate and quantify over 50 analytes, including multiple structural isomers of 3,3-HPHPA in human cerebrospinal fluid, serum and urine. Confirmation of identity was provided by NMR, by MS and other detection methods. The highly selective methods support rapid quantification of multiple metabolites and exhibit superior chromatographic behavior. CONCLUSION: An improved ultra-HPLC-MS/MS and structural approaches can accurately quantify 3,3-HPHPA and related analytes in human biological matrices.
AIM: Co-metabolism between a human host and the gastrointestinal microbiota generates many small phenolic molecules such as 3-hydroxy-3-(3-hydroxyphenyl)propanoic acid (3,3-HPHPA), which are reported to be elevated in schizophrenia and autism. Characterization of these chemicals, however, has been limited by analytic challenges. METHODOLOGY/ RESULTS: We applied HPLC to separate and quantify over 50 analytes, including multiple structural isomers of 3,3-HPHPA in human cerebrospinal fluid, serum and urine. Confirmation of identity was provided by NMR, by MS and other detection methods. The highly selective methods support rapid quantification of multiple metabolites and exhibit superior chromatographic behavior. CONCLUSION: An improved ultra-HPLC-MS/MS and structural approaches can accurately quantify 3,3-HPHPA and related analytes in human biological matrices.
Authors: Mark E Obrenovich; George E Jaskiw; Thriveen Sankar Chittoor Mana; Christina P Bennett; Jennifer Cadnum; Curtis J Donskey Journal: Pathog Immun Date: 2019-11-14
Authors: Anallely López-Yerena; Inés Domínguez-López; Anna Vallverdú-Queralt; Maria Pérez; Olga Jáuregui; Elvira Escribano-Ferrer; Rosa M Lamuela-Raventós Journal: Antioxidants (Basel) Date: 2021-05-25