Antioxidant systems responses and the compatible solutes as contributing factors to lead accumulation and tolerance in Lathyrus sativus inoculated by plant growth promoting rhizobacteria.

Short-term lead (Pb) uptake by plants is important to better understand the mechanisms of metal uptake, plant tolerance and detoxification strategy. Thus we examined the response of Lathyrus sativus to 1 mM Pb application in hydroponic sorption kinetics at 24, 48 and 72 h, and we investigated the contribution of two inocula I1 (R. leguminosarum (M5) + B. simplex + Luteibacter sp + Variovorax sp) and I5 (R. leguminosarum (M5) + P. fluorescens (K23) + Luteibacter sp + Variovorax sp) in plant mechanisms responses. Pb application induced its immediate uptake by L. sativus with highest concentrations, which increased gradually mostly for inoculated plants. The control plant shoots accumulated the highest concentration of lead at 24 h. However, at 48 and 72 h this potential uptake was significantly enhanced in plants inoculated with I5. Moreover, inoculation increased significantly root Pb-uptake with the maximum reached at 72 h. We observed a progressive decline in chlorophyll contents after Pb exposure in control plants that was higher than in PGPR-treated plants and the greatest improvement (152%) was recorded in I5 inoculated leaves. The PGPR also promoted significant elevation in the carotenoid content with the highest increases (188%) in plants inoculated with I5 at 72 h. Data illustrated remarkable augmentation in malondialdehyde, ion leakage level and decrease in membrane stability. Whereas, inoculation enhanced significantly cellular membrane integrity through increases in membrane stability index as compared to the control plants. In response to Pb, proline biosynthesis, as well as total soluble sugars concentration, immediately increased and the stimulatory effect was more pronounced in inoculated plants at 72 h. Lead considerably altered the activities of SOD, GPOX, CAT and APX enzymes in leaves and roots in a time- and inoculation- dependent manner. It is concluded that antioxidant enzymes, carotenoids, soluble sugars and proline were involved in the main defense mechanism and tolerance of Lathyrus sativus to Pb oxidative stress, as well lead accumulation, and are likely to operate in combination.