Huansheng Han1. 1. Harbin Specialty Research Institute, Heilongjiang Academy of Land Reclamation Sciences, No. 101 Xiangfu Road, Xiangfang District, Harbin 150038, China; Research and Development Center, Heilongjiang Puhui Specialty Co., LTD., Harbin 150038, China. Electronic address: HurryH_123@126.com.
Abstract
PURPOSE: This study was aimed at exploring the mechanisms and identifying the key candidate genes associated with S. aureus and E. coli mastitis. METHODS: A public microarray dataset GSE24560 was downloaded. Differentially expressed genes (DEGs) were screened in E. coli- and S. aureus-infected primary bovine mammary gland epithelial cell (pBMEC) samples, and compared with control samples at 1 h, 6 h, and 24 h. A functional enrichment analysis was performed, and construction of a gene co-expression network was performed based on genes that showed consistent changes over time, which were identified using time series expression analysis. Then, a miRNA/TF regulatory network was constructed based on the genes in the co-expression network. The genes in the miRNA/TF regulatory network were screened for involvement in related diseases. Furthermore, the expression of several selected DEGs was further validated using real-time RT-PCR. RESULTS: In total, 92 and 81 DEGs showed continuous differential expression over time in the E. coli- and S. aureus-inoculated groups. DEGs in the E. coli-inoculated group were associated with the RIG-I-like receptor signaling pathway, and those in the S. aureus-inoculated group were associated with the lysosome pathway. Time series expression analysis identified two gene clusters. NFKBIZ and GRO1 in the gene co-expression network were associated with inflammatory and defense responses. Moreover, several genes such as CXADR, APP, and CXCL2 in the miRNA/TF regulatory network, were associated with infection, inflammation, or stress-related diseases. CONCLUSION: RIG-I like receptor pathway and several DEGs such as NFKBIZ, GRO1, CXCL2, and CXADR may play critical roles in the response to infection in pBMECs.
PURPOSE: This study was aimed at exploring the mechanisms and identifying the key candidate genes associated with S. aureus and E. coli mastitis. METHODS: A public microarray dataset GSE24560 was downloaded. Differentially expressed genes (DEGs) were screened in E. coli- and S. aureus-infected primary bovine mammary gland epithelial cell (pBMEC) samples, and compared with control samples at 1 h, 6 h, and 24 h. A functional enrichment analysis was performed, and construction of a gene co-expression network was performed based on genes that showed consistent changes over time, which were identified using time series expression analysis. Then, a miRNA/TF regulatory network was constructed based on the genes in the co-expression network. The genes in the miRNA/TF regulatory network were screened for involvement in related diseases. Furthermore, the expression of several selected DEGs was further validated using real-time RT-PCR. RESULTS: In total, 92 and 81 DEGs showed continuous differential expression over time in the E. coli- and S. aureus-inoculated groups. DEGs in the E. coli-inoculated group were associated with the RIG-I-like receptor signaling pathway, and those in the S. aureus-inoculated group were associated with the lysosome pathway. Time series expression analysis identified two gene clusters. NFKBIZ and GRO1 in the gene co-expression network were associated with inflammatory and defense responses. Moreover, several genes such as CXADR, APP, and CXCL2 in the miRNA/TF regulatory network, were associated with infection, inflammation, or stress-related diseases. CONCLUSION: RIG-I like receptor pathway and several DEGs such as NFKBIZ, GRO1, CXCL2, and CXADR may play critical roles in the response to infection in pBMECs.