Danila de Oliveira1, Igor de Oliveira Puttini2, Pedro Henrique Silva Gomes-Ferreira2, Letícia Pitol Palin3, Mariza Akemi Matsumoto3, Roberta Okamoto3. 1. Department of Basic Sciences, Aracatuba Dental School, Univ. Estadual Paulista - UNESP, Rua Jose Bonifacio, n 1193, Aracatuba, Sao Paulo, 16015-050, Brazil. dani-oliveiraa@hotmail.com. 2. Department of Surgery and Integrated Clinic, Aracatuba Dental School, Univ. Estadual Paulista - UNESP, Aracatuba, Sao Paulo, Brazil. 3. Department of Basic Sciences, Aracatuba Dental School, Univ. Estadual Paulista - UNESP, Rua Jose Bonifacio, n 1193, Aracatuba, Sao Paulo, 16015-050, Brazil.
Abstract
OBJECTIVE: To evaluate intermittent teriparatide therapy's influence on the alveolar healing process in rats with induced osteoporosis. MATERIALS AND METHODS: Fifty-four male rats were divided into three groups: fictitious surgery (SHAM), bilateral orchiectomy (ORQ NT), and bilateral orchiectomy treated with teriparatide (ORQ TERI). Right upper incisor extraction was performed. After 14 and 42 days, the rats were euthanized. Immunolabeling analysis was performed in order to characterize bone turnover through Wnt, alcaline phosphatase, osteocalcin, and TRAP presence in tissue; micro-CT analysis was performed in order to determine the bone volume fraction (BV/TV), trabecular thickness, separation, and number (Tb.Th, Tb.Sp, Tb.N). For the micro-CT data, statistical analysis was performed through one-way ANOVA and Tukey post-test, for parametrical data, with significance level adopted in p < 0.05. Days, Wnt, alkaline phosphatase, and osteocalcin revealed more labeling for ORQ TERI and less for TRAP. For the Tb.N in the ORQ NT group was 0.496 mm, a significant statistical difference was observed between the groups of ORQ NT and ORQ TERI (p < 0.05). For the BV/TV, Tb.Sp, and Tb.Th parameters, no significant statistical difference was observed among the three experimental groups (p > 0.05). CONCLUSIONS: Treatment with intermittent teriparatide reverted impairment in the metabolism of repairing bone tissue in orchiectomized animals. CLINICAL RELEVANCE: Cases of decreased bone density such as osteoporosis can lead to delayed alveolar repair process. PTH 1-34 has been shown to be a medication that improves this repair, making bone of low quality into one with normal features.
<span class="abstract_title">OBJECTIVE: To evaluate intermittent <span class="Chemical">teriparatide therapy's influence on the alveolar healing process in rats with induced osteoporosis. MATERIALS AND METHODS: Fifty-four male rats were divided into three groups: fictitious surgery (SHAM), bilateral orchiectomy (ORQ NT), and bilateral orchiectomy treated with teriparatide (ORQ TERI). Right upper incisor extraction was performed. After 14 and 42 days, the rats were euthanized. Immunolabeling analysis was performed in order to characterize bone turnover through Wnt, alcaline phosphatase, osteocalcin, and TRAP presence in tissue; micro-CT analysis was performed in order to determine the bone volume fraction (BV/TV), trabecular thickness, separation, and number (Tb.Th, Tb.Sp, Tb.N). For the micro-CT data, statistical analysis was performed through one-way ANOVA and Tukey post-test, for parametrical data, with significance level adopted in p < 0.05. Days, Wnt, alkaline phosphatase, and osteocalcin revealed more labeling for ORQ TERI and less for TRAP. For the Tb.N in the ORQ NT group was 0.496 mm, a significant statistical difference was observed between the groups of ORQ NT and ORQ TERI (p < 0.05). For the BV/TV, Tb.Sp, and Tb.Th parameters, no significant statistical difference was observed among the three experimental groups (p > 0.05). CONCLUSIONS: Treatment with intermittent teriparatide reverted impairment in the metabolism of repairing bone tissue in orchiectomized animals. CLINICAL RELEVANCE: Cases of decreased bone density such as osteoporosis can lead to delayed alveolar repair process. PTH 1-34 has been shown to be a medication that improves this repair, making bone of low quality into one with normal features.
Authors: Carlos Henrique Bertoni Reis; Rogerio Leone Buchaim; Karina Torres Pomini; Abdul Latif Hamzé; Isabella Vasconcelos Zattiti; Marco Antonio Hungaro Duarte; Murilo Priori Alcalde; Benedito Barraviera; Rui Seabra Ferreira Júnior; Fenelon Martinho Lima Pontes; Carlos Roberto Grandini; Adriana de Cássia Ortiz; Simone Ortiz Moura Fideles; Renata Maria de Camargo Eugênio; Geraldo Marco Rosa Junior; Daniel de Bortoli Teixeira; Eliana de Souza Bastos Mazuqueli Pereira; João Paulo Galletti Pilon; Maria Angelica Miglino; Daniela Vieira Buchaim Journal: Polymers (Basel) Date: 2022-05-19 Impact factor: 4.967