Literature DB >> 3029012

Phenotypic changes and gene expression in human colon mucosal epithelial cells upon transfection of a SV40 DNA-gpt recombinant.

M P Moyer, J B Aust.   

Abstract

Phenotypic changes (increased longevity, decreased growth factor requirements, altered cell surface features, growth in semisolid agarose, and SV40 T antigen expression) suggesting in vitro transformation were displayed by human normal colon mucosal epithelial cells transfected with pSV3gpt, a pBR322 recombinant containing the SV40 "early" T antigen coding region and the dominant selectable marker bacterial gene, xanthine-guanine phosphoribosyltransferase. In contrast, control cultures which received neither DNA nor the recombinant pSV2gpt (which is identical to pSV3gpt but lacks the SV40 T antigen region) were not phenotypically altered.

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Year:  1987        PMID： 3029012     DOI： 10.1007/BF02623595

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol        ISSN： 0883-8364


  20 in total

1.  The action of oncogenes in the cytoplasm and nucleus.

Authors:  R A Weinberg
Journal:  Science       Date:  1985-11-15       Impact factor: 47.728

2.  A cancer-associated mucin alteration in benign colonic polyps.

Authors:  C R Boland; C K Montgomery; Y S Kim
Journal:  Gastroenterology       Date:  1982-04       Impact factor: 22.682

3.  Demonstration of infectious deoxyribonucleic acid in transformed cells. I. Recovery of simian virus 40 from yielder and nonyielder transformed cells.

Authors:  V A Boyd; J S Butel
Journal:  J Virol       Date:  1972-09       Impact factor: 5.103

4.  Alterations in human colonic mucin occurring with cellular differentiation and malignant transformation.

Authors:  C R Boland; C K Montgomery; Y S Kim
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

5.  Culture of human gastrointestinal epithelial cells.

Authors:  M P Moyer
Journal:  Proc Soc Exp Biol Med       Date:  1983-10

6.  Tissue culture of human epithelial cells from benign colonic tumors.

Authors:  E A Friedman; P J Higgins; M Lipkin; H Shinya; A M Gelb
Journal:  In Vitro       Date:  1981-07

7.  12-O-Tetradecanoylphorbol-13-acetate stimulation of DNA synthesis in cultured preneoplastic familial polyposis colonic epithelial cells but not in normal colonic epithelial cells.

Authors:  E Friedman; S Gillin; M Lipkin
Journal:  Cancer Res       Date:  1984-09       Impact factor: 12.701

8.  Colon epithelium. IV. Human colon carcinogenesis. Changes in human colon mucosa adjacent to and remote from carcinomas of the colon.

Authors:  A K Shamsuddin; L Weiss; P C Phelps; B F Trump
Journal:  J Natl Cancer Inst       Date:  1981-02       Impact factor: 13.506

9.  Glucagon enhances growth of cultured human colorectal cancer cells in vitro.

Authors:  M P Moyer; J B Aust; P S Dixon; B A Levine; K R Sirinek
Journal:  Am J Surg       Date:  1985-12       Impact factor: 2.565

10.  Disrupted communication between late-stage premalignant human colon epithelial cells by 12-O tetradecanoylphorbol-13-acetate.

Authors:  E A Friedman; M Steinberg
Journal:  Cancer Res       Date:  1982-12       Impact factor: 12.701
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  1 in total

1.  In vitro culture of human foetal colonic epithelial cells and their transformation with origin minus SV40 DNA.

Authors:  R D Berry; S C Powell; C Paraskeva
Journal:  Br J Cancer       Date:  1988-03       Impact factor: 7.640
  1 in total

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