Karina P Reis1,2,3, Laura E Sperling1,2, Cristian Teixeira1,2, Ágata Paim1,2, Bruno Alcântara1,2, Gema Vizcay-Barrena4, Roland A Fleck4, Patricia Pranke1,2,3,5. 1. Hematology & Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federale do Rio Grande do Sul, Porto Alegre, RS, 90610-000, Brazil. 2. Stem Cell Laboratory, Fundamental Health Science Institute, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil. 3. Post Graduate Program in Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil. 4. Centre for Ultrastructural Imaging, King's College London, London, WC2R 2LS, UK. 5. Stem Cell Research Institute, Porto Alegre, RS, 90020-10, Brazil.
Abstract
AIM: Scaffolds are a promising approach for spinal cord injury (SCI) treatment. FGF-2 is involved in tissue repair but is easily degradable and presents collateral effects in systemic administration. In order to address the stability issue and avoid the systemic effects, FGF-2 was encapsulated into core-shell microfibers by coaxial electrospinning and its in vitro and in vivo potential were studied. Materials & methods: The fibers were characterized by physicochemical and biological parameters. The scaffolds were implanted in a hemisection SCI rat model. Locomotor test was performed weekly for 6 weeks. After this time, histological analyses were performed and expression of nestin and GFAP was quantified by flow cytometry. Results: Electrospinning resulted in uniform microfibers with a core-shell structure, with a sustained liberation of FGF-2 from the fibers. The fibers supported PC12 cells adhesion and proliferation. Implanted scaffolds into SCI promoted locomotor recovery at 28 days after injury and reduced GFAP expression. CONCLUSION: These results indicate the potential of these microfibers in SCI tissue engineering. [Formula: see text].
AIM: Scaffolds are a promising approach for spinal cord injury (SCI) treatment. FGF-2 is involved in tissue repair but is easily degradable and presents collateral effects in systemic administration. In order to address the stability issue and avoid the systemic effects, FGF-2 was encapsulated into core-shell microfibers by coaxial electrospinning and its in vitro and in vivo potential were studied. Materials & methods: The fibers were characterized by physicochemical and biological parameters. The scaffolds were implanted in a hemisection SCI rat model. Locomotor test was performed weekly for 6 weeks. After this time, histological analyses were performed and expression of nestin and GFAP was quantified by flow cytometry. Results: Electrospinning resulted in uniform microfibers with a core-shell structure, with a sustained liberation of FGF-2 from the fibers. The fibers supported PC12 cells adhesion and proliferation. Implanted scaffolds into SCI promoted locomotor recovery at 28 days after injury and reduced GFAP expression. CONCLUSION: These results indicate the potential of these microfibers in SCI tissue engineering. [Formula: see text].
Authors: K P Reis; L E Sperling; C Teixeira; L Sommer; M Colombo; L S Koester; P Pranke Journal: Braz J Med Biol Res Date: 2020-04-09 Impact factor: 2.590