J Zhang1,2,3, E T Wang4, R P Singh5,6, C Guo2, Y Shang2, J Chen1, C Liu1. 1. Grape Diversity and Resources, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, China. 2. Department of Bioengineering and Biotechnology, College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, China. 3. Environmental Microbiology, Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou, Henan Province, China. 4. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D. F., México. 5. Microbial Genomics Laboratory, National Bureau of Agriculturally Important Microorganism, Maunath Bhanjan, Uttar Pradesh, India. 6. Department of Research and Development, Uttaranchal University, Dehradun, India.
Abstract
AIMS: Bacterial microbiome on grape berry surface may play an important role in grape quality and health. This study aims to investigate the impact of grape varieties and clones on grape berry surface bacterial microbiome from the same vineyard. METHODS AND RESULTS: High-throughput sequencing strategy was used to investigate the bacterial diversity and abundance on the grape surfaces of 12 clones belonging to six varieties grown in the same vineyard of Zhengzhou Fruit Research Institute in Henan Province. In total, 45 bacterial phyla and 933 genera were detected from all samples. Cyanobacteria, Proteobacteria and Firmicutes were the most abundant and prevalent phyla, while Bacteroidetes, Chloroflexi, Acidobacteria and Planctomycetes were grape clone specific phyla. The nonrank genus from phylum Cyanobacteria occupied 30-81% of grape clones from Italian Riesling (GRX), Cabernet Franc (PLZ), Pinot Blanc (BBN) and Riesling (LSL). Interestingly, Bacillus, Pseudomonas and Lactococcus were the only three prevalent genera found on all the clones. Furthermore, the predicted functional activities of grape surface bacterial communities varied according to the clones. CONCLUSIONS: The present study revealed that in addition to the grape varieties, the variations in grape clone background may also affect the bacterial microbiome on grape surfaces which may ultimately determine their functional activities. SIGNIFICANCE AND IMPACT OF THE STUDY: This research provides an important information for grape planting and wine fermentation that not only the grape varieties need to be paid attention but also grape clones from the specific variety need to be concerned.
AIMS: Bacterial microbiome on grape berry surface may play an important role in grape quality and health. This study aims to investigate the impact of grape varieties and clones on grape berry surface bacterial microbiome from the same vineyard. METHODS AND RESULTS: High-throughput sequencing strategy was used to investigate the bacterial diversity and abundance on the grape surfaces of 12 clones belonging to six varieties grown in the same vineyard of Zhengzhou Fruit Research Institute in Henan Province. In total, 45 bacterial phyla and 933 genera were detected from all samples. Cyanobacteria, Proteobacteria and Firmicutes were the most abundant and prevalent phyla, while Bacteroidetes, Chloroflexi, Acidobacteria and Planctomycetes were grape clone specific phyla. The nonrank genus from phylum Cyanobacteria occupied 30-81% of grape clones from Italian Riesling (GRX), Cabernet Franc (PLZ), Pinot Blanc (BBN) and Riesling (LSL). Interestingly, Bacillus, Pseudomonas and Lactococcus were the only three prevalent genera found on all the clones. Furthermore, the predicted functional activities of grape surface bacterial communities varied according to the clones. CONCLUSIONS: The present study revealed that in addition to the grape varieties, the variations in grape clone background may also affect the bacterial microbiome on grape surfaces which may ultimately determine their functional activities. SIGNIFICANCE AND IMPACT OF THE STUDY: This research provides an important information for grape planting and wine fermentation that not only the grape varieties need to be paid attention but also grape clones from the specific variety need to be concerned.
Authors: C G Conacher; N A Luyt; R K Naidoo-Blassoples; D Rossouw; M E Setati; F F Bauer Journal: Appl Microbiol Biotechnol Date: 2021-04-09 Impact factor: 4.813
Authors: Kerri L Steenwerth; Ian Morelan; Ruby Stahel; Rosa Figueroa-Balderas; Dario Cantu; Jungmin Lee; Ron C Runnebaum; Amisha T Poret-Peterson Journal: PeerJ Date: 2021-02-04 Impact factor: 2.984