Literature DB >> 3028842

Differences between rodent and human cell lines in the amount of integrated DNA after transfection.

J H Hoeijmakers, H Odijk, A Westerveld.   

Abstract

The suitability of Chinese hamster and human cell lines for DNA-mediated gene transformation was investigated with respect to two parameters: the average quantity of and the integrity of integrated exogenous DNA fragments. No large differences were observed between most cell lines concerning the extent of fragmentation of the transferred DNA molecules. By contrast, the average number of sequences stably incorporated by the human cells (four lines tested) was 20- to 100-fold lower than the average amount inserted in the five Chinese hamster lines investigated. The very low uptake exhibited by the human cells, ranging from less than 100 up to 500 kb, renders these cells less suitable for transfection with genomic DNA to isolate specific genes.

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Year:  1987        PMID： 3028842     DOI： 10.1016/0014-4827(87)90230-8

Source DB:  PubMed          Journal:  Exp Cell Res        ISSN： 0014-4827            Impact factor:   3.905


  24 in total

1.  Functional complementation of ataxia-telangiectasia group D (AT-D) cells by microcell-mediated chromosome transfer and mapping of the AT-D locus to the region 11q22-23.

Authors:  C Lambert; R A Schultz; M Smith; C Wagner-McPherson; L D McDaniel; T Donlon; E J Stanbridge; E C Friedberg
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

2.  Cloning of a candidate gene for ataxia-telangiectasia group D.

Authors:  L N Kapp; R B Painter; L C Yu; N van Loon; C W Richard; M R James; D R Cox; J P Murnane
Journal:  Am J Hum Genet       Date:  1992-07       Impact factor: 11.025

3.  Substantial narrowing of the Niemann-Pick C candidate interval by yeast artificial chromosome complementation.

Authors:  J Z Gu; E D Carstea; C Cummings; J A Morris; S K Loftus; D Zhang; K G Coleman; A M Cooney; M E Comly; L Fandino; C Roff; D A Tagle; W J Pavan; P G Pentchev; M A Rosenfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-08       Impact factor: 11.205

4.  Molecular cloning of the human DNA excision repair gene ERCC-6.

Authors:  C Troelstra; H Odijk; J de Wit; A Westerveld; L H Thompson; D Bootsma; J H Hoeijmakers
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

5.  Recombination events during integration of transfected DNA into normal human cells.

Authors:  J P Murnane; M J Yezzi; B R Young
Journal:  Nucleic Acids Res       Date:  1990-05-11       Impact factor: 16.971

6.  Molecular cloning and characterization of a mammalian excision repair gene that partially restores UV resistance to xeroderma pigmentosum complementation group D cells.

Authors:  J E Arrand; N M Bone; R T Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

7.  Molecular cloning and biological characterization of the human excision repair gene ERCC-3.

Authors:  G Weeda; R C van Ham; R Masurel; A Westerveld; H Odijk; J de Wit; D Bootsma; A J van der Eb; J H Hoeijmakers
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

8.  Expression cloning of multiple human cDNAs that complement the phenotypic defects of ataxia-telangiectasia group D fibroblasts.

Authors:  M S Meyn; J M Lu-Kuo; L B Herzing
Journal:  Am J Hum Genet       Date:  1993-12       Impact factor: 11.025

9.  Structure and expression of the human XPBC/ERCC-3 gene involved in DNA repair disorders xeroderma pigmentosum and Cockayne's syndrome.

Authors:  G Weeda; L B Ma; R C van Ham; A J van der Eb; J H Hoeijmakers
Journal:  Nucleic Acids Res       Date:  1991-11-25       Impact factor: 16.971

10.  Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT-) using an Epstein-Barr virus-derived cDNA expression vector.

Authors:  P B Belt; W Jongmans; J de Wit; J H Hoeijmakers; P van de Putte; C Backendorf
Journal:  Nucleic Acids Res       Date:  1991-09-25       Impact factor: 16.971
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