Literature DB >> 30286169

Correction: Analytical Sensitivity Comparison between Singleplex Real-Time PCR and a Multiplex PCR Platform for Detecting Respiratory Viruses.

Jayme Parker, Nisha Fowler, Mary Louise Walmsley, Terri Schmidt, Jason Scharrer, James Kowaleski, Teresa Grimes, Shanann Hoyos, Jack Chen.   

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0143164.].

Entities:  

Year:  2018        PMID: 30286169      PMCID: PMC6171942          DOI: 10.1371/journal.pone.0205483

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


There are errors in Table 3. The LOD reported for VR-92, VR-1540, and VR-955 is incorrect. Please see the corrected Table 3 here.
Table 3

Relationship between TCID50/mL concentrations and copy number.

ATCC CultureGenome Copies/TCID50 (± SD)LOD for GenMark eSensor assays (TCID50/mL)
VR-1Adenovirus Type 1 (C)7 ± 18.89 x 101
VR-1572Adenovirus Type 4 (E)124 ± 141.58 x 101
VR-547Influenza A/H3 (Aichi)0.01 ± 01.58 x 103
VR-1736Influenza A/H1N12,381 ± 1,0481.05 x 10−1
VR-101Influenza B16 ± 443.16 x 10−1
VR-94Human Parainfluenza Virus Type 1 (C35)391 ± 02.81 x 10−2
VR-92Human Parainfluenza Virus Type 2 (Greer)0.03 ± 0.012.81 x 100
VR-93Human Parainfluenza Virus Type 3 (C243)24 ± 12.81 x 101
VR-1540Respiratory Syncytial Virus (A2)6 ± 12.81 x 100
VR-955Respiratory Syncytial Virus (B9320)38 ± 31.58 x 100
VR-483Rhinovirus 3 FEB53,797 ± 01.58 x 10−3

SD = standard deviation, SD could not be calculated for VR-547, VR-94, and VR-483 since the TCID50/mL concentration exceeded the detection limit on the qPCR assay.

SD = standard deviation, SD could not be calculated for VR-547, VR-94, and VR-483 since the TCID50/mL concentration exceeded the detection limit on the qPCR assay.
  1 in total

1.  Analytical Sensitivity Comparison between Singleplex Real-Time PCR and a Multiplex PCR Platform for Detecting Respiratory Viruses.

Authors:  Jayme Parker; Nisha Fowler; Mary Louise Walmsley; Terri Schmidt; Jason Scharrer; James Kowaleski; Teresa Grimes; Shanann Hoyos; Jack Chen
Journal:  PLoS One       Date:  2015-11-16       Impact factor: 3.240

  1 in total

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