Braham D Arya1,2, Sandeep Mittal2,3, Prachi Joshi1, Alok K Pandey2,3, Jaime E Ramirez-Vick4, Surinder P Singh1,2. 1. CSIR-National Physical Laboratory, Dr K. S. Krishnan Marg, New Delhi 110012, India. 2. Academy of Scientific & Innovative Research (AcSIR), Sector 19, Ghaziabad, UP 201002, India. 3. CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31, Mahatma Gandhi Marg, Lucknow 226001, India. 4. Department of Biomedical, Industrial & Human Factors Engineering, Wright State University, Dayton, OH 45435, USA.
Abstract
AIM: Chloroquine (Chl) has shown its potential in cancer therapy and graphene oxide (GO) exhibited excellent tumor-targeting ability, biocompatibility and low toxicity. We have endeavored to conjugate Chl to GO sheets and investigated the nonproliferation action on A549 cell lines along with cell signaling pathways. MATERIALS & METHODS: Cellular toxicity, autophagic flux modulation and cell death mechanism induced by GO-Chl have been investigated on A549 cell lines. RESULTS & CONCLUSION: GO-Chl induces accumulation of autophagosomes (monodansylcadaverine staining, green fluorescence protein-tagged LC3 plasmid and transmission electron microscopy observations) in A549 cells through the blockade of autophagic flux that serves as scaffold for necrosome assembling and activates necroptotic cell death. GO-Chl nanoconjugate could be used as an effective cancer therapeutic agent, by targeting the autophagy necroptosis axis.
AIM: Chloroquine (Chl) has shown its potential in cancer therapy and graphene oxide (GO) exhibited excellent tumor-targeting ability, biocompatibility and low toxicity. We have endeavored to conjugate Chl to GO sheets and investigated the nonproliferation action on A549 cell lines along with cell signaling pathways. MATERIALS & METHODS: Cellular toxicity, autophagic flux modulation and cell death mechanism induced by GO-Chl have been investigated on A549 cell lines. RESULTS & CONCLUSION:GO-Chl induces accumulation of autophagosomes (monodansylcadaverine staining, green fluorescence protein-tagged LC3 plasmid and transmission electron microscopy observations) in A549 cells through the blockade of autophagic flux that serves as scaffold for necrosome assembling and activates necroptotic cell death. GO-Chl nanoconjugate could be used as an effective cancer therapeutic agent, by targeting the autophagy necroptosis axis.
Authors: Ping Liu; Chengbin Ma; Qiongwei Wu; Wenying Zhang; Cao Wang; Li Yuan; Xiaowei Xi Journal: Cancer Cell Int Date: 2019-07-11 Impact factor: 5.722
Authors: Rong Tang; Jin Xu; Bo Zhang; Jiang Liu; Chen Liang; Jie Hua; Qingcai Meng; Xianjun Yu; Si Shi Journal: J Hematol Oncol Date: 2020-08-10 Impact factor: 17.388