Literature DB >> 3028408

Cloning and regulation of rat apolipoprotein B mRNA.

A Matsumoto, H Aburatani, Y Shibasaki, T Kodama, F Takaku, H Itakura.   

Abstract

Recombinant cDNA clones that code for apolipoprotein B(apoB) were isolated from a rat liver cDNA library, using synthetic oligonucleotide probe derived from the sequence of human apoB cDNA. The nucleotide and deduced amino acid sequences of the rat apoB clone pRB5, 1.2 kb in length, showed 83% and 84% homology to those of human apoB. Northern blot analysis revealed that rat apoB cDNA probe cross-reacts with human and rabbit apoB mRNA sequences and the size of those mRNAs, approximately 15 kb long, were not discernibly different. In addition, apoB mRNA was abundant only in the liver and intestine. Finally, cholesterol feeding to rats for six weeks resulted in a several-fold increase in the level of apoB mRNA in the liver.

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Year:  1987        PMID： 3028408     DOI： 10.1016/0006-291x(87)90455-4

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  5 in total

1.  Plasma lipoprotein metabolism in transgenic mice overexpressing apolipoprotein E. Accelerated clearance of lipoproteins containing apolipoprotein B.

Authors:  H Shimano; N Yamada; M Katsuki; K Yamamoto; T Gotoda; K Harada; M Shimada; Y Yazaki
Journal:  J Clin Invest       Date:  1992-11       Impact factor: 14.808

2.  Very low density lipoprotein secretion by cultured hepatocytes of rabbits fed purified or autoxidized cholesterol.

Authors:  V A Kosykh; V Z Lankin; E A Podrez; D K Novikov; S A Volgushev; A V Victorov; V S Repin; V N Smirnov
Journal:  Lipids       Date:  1989-02       Impact factor: 1.880

3.  Control of Hep G2-cell triacylglycerol and apolipoprotein B synthesis and secretion by polyunsaturated non-esterified fatty acids and insulin.

Authors:  C D Byrne; T W Wang; C N Hales
Journal:  Biochem J       Date:  1992-11-15       Impact factor: 3.857

4.  Overproduction of cholesterol and fatty acids causes massive liver enlargement in transgenic mice expressing truncated SREBP-1a.

Authors:  H Shimano; J D Horton; R E Hammer; I Shimomura; M S Brown; J L Goldstein
Journal:  J Clin Invest       Date:  1996-10-01       Impact factor: 14.808

5.  Modulation of hepatic apolipoprotein B, 3-hydroxy-3-methylglutaryl-CoA reductase and low-density lipoprotein receptor mRNA and plasma lipoprotein concentrations by defined dietary fats. Comparison of trimyristin, tripalmitin, tristearin and triolein.

Authors:  A J Bennett; M A Billett; A M Salter; E H Mangiapane; J S Bruce; K L Anderton; C B Marenah; N Lawson; D A White
Journal:  Biochem J       Date:  1995-10-01       Impact factor: 3.857
  5 in total

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