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Literature DB >> 3028336

In vitro translation of mRNA species from cells infected with Machupo virus.

I S Lukashevich, T A Stelmakh, E P Stchesljenok, T V Shkolina.

Abstract

RNA from Machupo virus infected cells was centrifuged in a linear sucrose gradient and RNAs from gradient fractions were tested separately for template activity in a cell-free protein synthesizing system from rabbit reticulocytes. Fraction 15-16 S programmed the synthesis of protein that migrated in SDS-polyacrylamide gel like the nucleocapsid protein of a purified virus. The synthesis of virus glycoproteins was not detected in the system.

Entities: Chemical Species

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Year: 1987 PMID： 3028336 DOI： 10.1007/BF01317488

Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574

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References

11 in total

1. Coupled in vitro transcription and translation of vesicular stomatitis virus messenger RNA.

Authors: M Breindl; J J Holland
Journal: Proc Natl Acad Sci U S A Date: 1975-07 Impact factor: 11.205

2. Translation and identification of the mRNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus.

Authors: G W Both; S A Moyer; A K Banerjee
Journal: Proc Natl Acad Sci U S A Date: 1975-01 Impact factor: 11.205

In vitro translation of mRNA species from cells infected with Machupo virus.

1. Coupled in vitro transcription and translation of vesicular stomatitis virus messenger RNA.

2. Translation and identification of the mRNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus.

3. L and S species of Machupo virion RNA contain a mixture of complementary strands. Brief report.

4. Structural polypeptides of Machupo virus.

5. An efficient mRNA-dependent translation system from reticulocyte lysates.

6. Ribonucleic acids of Machupo and Lassa viruses.

7. The sequences of the N protein gene and intergenic region of the S RNA of pichinde arenavirus.

8. Translation of vesicular stomatitis messenger RNA by extracts from mammalian and plant cells.

9. Machupo virus polypeptides: identification by immunoprecipitation.

10. Sequencing studies of pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA.