Literature DB >> 3028208

Separation of phosphoinositides and other phospholipids by two-dimensional thin-layer chromatography.

K T Mitchell, J E Ferrell, W H Huestis.   

Abstract

A simple, rapid, two-dimensional TLC system is presented which resolves the four phosphoinositide cycle phospholipids as well as all commonly encountered major and minor phospholipids. Ca2+-free lipid samples are loaded onto silica gel HL plates and developed first in 48:40:7:5 chloroform:methanol:water:concentrated ammonia, and then in 55:25:5 chloroform:methanol:formic acid. The method was applied successfully to human erythrocytes, human platelets, and BL/VL3 murine lymphoma cells.

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Year:  1986        PMID： 3028208     DOI： 10.1016/0003-2697(86)90574-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  17 in total

1.  The F-BAR domains from srGAP1, srGAP2 and srGAP3 regulate membrane deformation differently.

Authors:  Jaeda Coutinho-Budd; Vladimir Ghukasyan; Mark J Zylka; Franck Polleux
Journal:  J Cell Sci       Date:  2012-03-30       Impact factor: 5.285

2.  Marked enrichment of the alkenylacyl subclass of plasma ethanolamine glycerophospholipid with eicosapentaenoic acid in human subjects consuming a fish oil concentrate.

Authors:  T Wilkinson; H M Aukema; L M Thomas; B J Holub
Journal:  Lipids       Date:  1996-03       Impact factor: 1.880

3.  Methylmalonic acid metabolism and nervous-system fatty acids in cobalamin-deficient fruit bats receiving supplements of methionine, valine and isoleucine.

Authors:  E Vieira-Makings; N Chetty; S C Reavis; J Metz
Journal:  Biochem J       Date:  1991-05-01       Impact factor: 3.857

4.  Restricted diffusion of integral membrane proteins and polyphosphoinositides leads to their depletion in microvesicles released from human erythrocytes.

Authors:  C Hagelberg; D Allan
Journal:  Biochem J       Date:  1990-11-01       Impact factor: 3.857

5.  Albumin-bound docosahexaenoic acid and collagen-induced human platelet reactivity.

Authors:  D C Gaudette; B J Holub
Journal:  Lipids       Date:  1990-03       Impact factor: 1.880

6.  Evidence that the inositol phospholipids are necessary for exocytosis. Loss of inositol phospholipids and inhibition of secretion in permeabilized cells caused by a bacterial phospholipase C and removal of ATP.

Authors:  D A Eberhard; C L Cooper; M G Low; R W Holz
Journal:  Biochem J       Date:  1990-05-15       Impact factor: 3.857

7.  Regulation of the formation of inositol phosphates by calcium, guanine nucleotides and ATP in digitonin-permeabilized bovine adrenal chromaffin cells.

Authors:  D A Eberhard; R W Holz
Journal:  Biochem J       Date:  1991-10-15       Impact factor: 3.857

8.  Calcium-independent effects of TMB-8. Modification of phospholipid metabolism in neuroblastoma cells by inhibition of choline uptake.

Authors:  F B Palmer; D M Byers; M W Spence; H W Cook
Journal:  Biochem J       Date:  1992-09-01       Impact factor: 3.857

9.  The role of diacylglycerol in the exocytosis of the sperm acrosome. Studies using diacylglycerol lipase and diacylglycerol kinase inhibitors and exogenous diacylglycerols.

Authors:  E R Roldan; R A Harrison
Journal:  Biochem J       Date:  1992-02-01       Impact factor: 3.857

10.  Carbachol stimulation of phospholipase A2 and insulin secretion in pancreatic islets.

Authors:  R J Konrad; Y C Jolly; C Major; B A Wolf
Journal:  Biochem J       Date:  1992-10-01       Impact factor: 3.857
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