| Literature DB >> 30276745 |
Abstract
Targeted proteomics detects proteins of interest with high sensitivity, quantitative accuracy, and reproducibility. In a targeted proteomics assay, surrogate peptides are generated by proteolytic digestion of target proteins and selected reaction monitoring (SRM) assays are developed to quantify these peptides using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this report, we describe the details of quantitative analysis of target protein in cells and tissue samples.Keywords: Cells and tissue samples; Liquid chromatography-tandem mass spectrometry; Protein quantification; Targeted proteomics
Mesh：
Substances：
Year: 2019 PMID： 30276745 DOI： 10.1007/978-1-4939-8814-3_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745