Determination of proper treatment time for in vivo blood coagulation and wound healing application by non-thermal helium plasma jet.

The aim of this research was to determine the time of blood coagulation of in vivo cuts that treated by the non-thermal atmospheric pressure plasma jet. Also, the effect of different treatment times on wound healing has been studied. The non-thermal atmospheric pressure plasma jet working in helium gas has been used. The averaged treatment time of 8.6 s for in vivo cuts on the Balb/c mouse liver showed the complete blood coagulation. Also, the effect of tretament time on wound healing has been studied by applying plasma on the wounds for different times (10, 20, 30, 40 and 50 s). It was obtained from morphological analysis that the treatment groups of 30 s, 40 s and 50 s cause the wounds to be healed faster than the groups 10 s and 20 s.The histological analysis showed that in 30 s and 40 s treatment time groups, the repair process of treated wounds has been accelerated, while for 50 s group, it has not been completed, yet. The 30 s treatment time has been chosen because of imposing lower dose to living tissue. The treated wound area reduction ratios against the control wound reduction ratios for 30 s group were obtained 78%, 77% and 63% at the 3rd, 5th and 8th days, respectively.