Patricio Gonzalez-Hormazabal1, Sandra Romero2, Maher Musleh3,4, Marco Bustamante5, Juan Stambuk6, Raul Pisano7, Enrique Lanzarini3,4, Hector Chiong8, Jorge Rojas4, Victor Gonzalo Castro2, Lilian Jara2, Zoltan Berger3. 1. Human Genetics Program, Institute of Biomedical Sciences (ICBM), School of Medicine, University of Chile, Santiago, Chile patriciogonzalez@uchile.cl. 2. Human Genetics Program, Institute of Biomedical Sciences (ICBM), School of Medicine, University of Chile, Santiago, Chile. 3. Department of Gastroenterology, University of Chile Clinical Hospital, Santiago, Chile. 4. Department of Surgery, University of Chile Clinical Hospital, Santiago, Chile. 5. Department of Surgery, School of Medicine at Eastern Campus, University of Chile, Santiago, Chile. 6. Department of Surgery, San Juan de Dios Hospital, Santiago, Chile. 7. Department of Pathology, San Juan de Dios Hospital, Santiago, Chile. 8. Department of Surgery, Barros Luco Hospital, Santiago, Chile.
Abstract
BACKGROUND/AIM: Inflammation is a key process in gastric carcinogenesis. Cytokines are mediators of inflammation and are involved in metastasis and tumorigenicity. We previously assessed the role of cytokine gene polymorphisms in gastric cancer risk in Chile. In the present study, we aimed to analyze whether these polymorphisms are associated with overall survival (OS) in gastric cancer (GC) patients. PATIENTS AND METHODS: A total of 153 individuals with GC diagnosis were followed-up for at least 2 years. Hazard ratios (HR) were estimated from Cox regression models using SNPs as predictor variables. The following SNPs were genotyped for study using a TaqMan assay: rs16944 (IL1B -511C>T); rs4073 (IL8 -251 T>A); rs2275913 (IL-17 -197G>A); rs1800872 (IL10 -592 C>A); rs1800896 (IL10 -1082A>G); rs28372698 (IL32). RESULTS: Interleukin-8 rs4073 (IL-8 -251T>A) showed association with OS under the dominant model (TA + AA) only when adjusted by clinicopathological variables (HR=1.64, 95%CI=1.05-2.55, p=0.030, q-value=0.18), but not with the univariate model (HR=1.51, 95%CI=0.98-2.31, p=0.062, q-value=0.37). No significant differences were observed after adjusting for population stratification (PC1 and PC2 from Principal Component Analysis using genotypes from Infinium Global Screening Array). After stratification by clinicopathological variables, the association with shorter overall survival was higher among patients with diffuse-type tumors (HR=2.24, 95%CI=1.16-4.45) and patients with tumor size >5 cm (HR=1.79, 95%CI=1.08-2.97). CONCLUSION: These results suggest a role of IL-8 rs4073 in cancer prognosis. Its use as a prognostic marker of GC survival warrants further investigation. Copyright
BACKGROUND/AIM: Inflammation is a key process in gastric carcinogenesis. Cytokines are mediators of inflammation and are involved in metastasis and tumorigenicity. We previously assessed the role of cytokine gene polymorphisms in gastric cancer risk in Chile. In the present study, we aimed to analyze whether these polymorphisms are associated with overall survival (OS) in gastric cancer (GC) patients. PATIENTS AND METHODS: A total of 153 individuals with GC diagnosis were followed-up for at least 2 years. Hazard ratios (HR) were estimated from Cox regression models using SNPs as predictor variables. The following SNPs were genotyped for study using a TaqMan assay: rs16944 (IL1B -511C>T); rs4073 (IL8 -251 T>A); rs2275913 (IL-17 -197G>A); rs1800872 (IL10 -592 C>A); rs1800896 (IL10 -1082A>G); rs28372698 (IL32). RESULTS:Interleukin-8rs4073 (IL-8 -251T>A) showed association with OS under the dominant model (TA + AA) only when adjusted by clinicopathological variables (HR=1.64, 95%CI=1.05-2.55, p=0.030, q-value=0.18), but not with the univariate model (HR=1.51, 95%CI=0.98-2.31, p=0.062, q-value=0.37). No significant differences were observed after adjusting for population stratification (PC1 and PC2 from Principal Component Analysis using genotypes from Infinium Global Screening Array). After stratification by clinicopathological variables, the association with shorter overall survival was higher among patients with diffuse-type tumors (HR=2.24, 95%CI=1.16-4.45) and patients with tumor size >5 cm (HR=1.79, 95%CI=1.08-2.97). CONCLUSION: These results suggest a role of IL-8rs4073 in cancer prognosis. Its use as a prognostic marker of GC survival warrants further investigation. Copyright
Authors: Dennis Cerrato-Izaguirre; Yolanda I Chirino; Claudia M García-Cuellar; Miguel Santibáñez-Andrade; Diddier Prada; Angélica Hernández-Guerrero; Octavio Alonso Larraga; Javier Camacho; Yesennia Sánchez-Pérez Journal: Genes Dis Date: 2021-04-24
Authors: Silvia Selene Moreno-Guerrero; Arturo Ramírez-Pacheco; Luz María Rocha-Ramírez; Gabriela Hernández-Pliego; Pilar Eguía-Aguilar; María Argelia Escobar-Sánchez; Alfonso Reyes-López; Luis Enrique Juárez-Villegas; Juan José Luis Sienra-Monge Journal: Cancers (Basel) Date: 2021-01-30 Impact factor: 6.639