Hai-Tao Yu1, Xiao-Yi Fu1, Bin Xu1, Li-Li Zuo1, Hong-Bo Ma1, Shu-Ran Wang2. 1. School of Public Health, Jilin Medical University, Jilin, China. 2. School of Public Health, Jilin Medical University, Jilin, China. shuranwang2014@163.com.
Abstract
BACKGROUND AND OBJECTIVES: Obesity is linked to metabolic diseases characterized by insulin resistance, such as diabetes and cardiovascular disease. In this study, we investigated the metabolic disorders of uncomplicated obesity to identify early alterations in biological systems. METHODS AND STUDY DESIGN: Metabolic differences between overweight/obese (n=36) and normal-weight (n=35) young Chinese men without known metabolic disorders were assessed. Metabolic profiling of the serum and urine was performed using ultra-performance liquidchromatography quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Partial least squares discriminant analysis (PLS-DA) was undertaken to reveal and classify the differences between the two groups. RESULTS: Compared to normal-weight men, obese men had higher levels of the serum metabolites phenylalanine, Phe-Phe, and L-tryptophan, whereas those of p-cresol sulfate and p-cresol were less in obesity. Urinary metabolites phenylacetamide, L-glutamine, phenylacetylglutamine, indoxyl sulfate, p-cresol, and p-cresol sulfate were greater in obese men. CONCLUSIONS: These findings indicate that disorders involving aromatic amino acids and the tricarboxylic acid cycle (TCA) have microbiomic involvement in the uncomplicated phase of obesity.
BACKGROUND AND OBJECTIVES:Obesity is linked to metabolic diseases characterized by insulin resistance, such as diabetes and cardiovascular disease. In this study, we investigated the metabolic disorders of uncomplicated obesity to identify early alterations in biological systems. METHODS AND STUDY DESIGN: Metabolic differences between overweight/obese (n=36) and normal-weight (n=35) young Chinese men without known metabolic disorders were assessed. Metabolic profiling of the serum and urine was performed using ultra-performance liquidchromatography quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Partial least squares discriminant analysis (PLS-DA) was undertaken to reveal and classify the differences between the two groups. RESULTS: Compared to normal-weight men, obesemen had higher levels of the serum metabolites phenylalanine, Phe-Phe, and L-tryptophan, whereas those of p-cresol sulfate and p-cresol were less in obesity. Urinary metabolites phenylacetamide, L-glutamine, phenylacetylglutamine, indoxyl sulfate, p-cresol, and p-cresol sulfate were greater in obesemen. CONCLUSIONS: These findings indicate that disorders involving aromatic amino acids and the tricarboxylic acid cycle (TCA) have microbiomic involvement in the uncomplicated phase of obesity.
Authors: Rocío Quiroga; Esther Nistal; Brisamar Estébanez; David Porras; María Juárez-Fernández; Susana Martínez-Flórez; María Victoria García-Mediavilla; José A de Paz; Javier González-Gallego; Sonia Sánchez-Campos; María J Cuevas Journal: Exp Mol Med Date: 2020-07-06 Impact factor: 8.718
Authors: Exsal M Albores-Mendez; Alexis D Aguilera Hernández; Alejandra Melo-González; Marco A Vargas-Hernández; Neptalí Gutierrez de la Cruz; Miguel A Vazquez-Guzman; Melchor Castro-Marín; Pablo Romero-Morelos; Robert Winkler Journal: PeerJ Date: 2022-07-22 Impact factor: 3.061