Specific purification of monoclonal anti-DNA antibodies from culture medium using a DNA-coupled Sepharose 4B affinity column.

Human monoclonal antibodies against DNA were specifically purified from the culture medium of an EBV transformant of SLE patients' lymphocytes using a DNA-coupled Sepharose 4B affinity column. The monoclonal antibodies were eluted from the column with 5% dimethylsulfoxide (pH 10.7) containing 0.5 M NaCl without loss of immunological activity and without contamination by other proteins.