Manal S Fawzy1,2, Baraah T Abu AlSel3, Essam Al Ageeli4, Saeed Awad Al-Qahtani5, Mohamed M Abdel-Daim6, Eman A Toraih7,8. 1. Department of Biochemistry, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia. 2. Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. 3. Department of Microbiology, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia. 4. Department of Clinical Biochemistry (Medical Genetics), Faculty of Medicine, Jazan University, Jazan, Saudi Arabia. 5. Department of Physiology, Faculty of Medicine, Taibah University, Almadinah Almunawwarah, Saudi Arabia. 6. Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. 7. Department of Histology and Cell Biology (Genetics Unit), Faculty of Medicine, Suez Canal University, Ismailia, Egypt. 8. Center of Excellence of Molecular and Cellular Medicine, Suez Canal University, Ismailia, Egypt.
Abstract
Background: Circulating non-coding RNAs (ncRNAs) have been implicated in health and disease. This study aimed to evaluate the serum expression profile of microRNA-499a (miR-499a) and its selected bioinformatically predicted partner long-ncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) in diabetes-related end-stage renal disease (ESRD) patients and to correlate the expressions with the patients' clinicolaboratory data.Subjects and methods: Real-time quantitative polymerase chain reaction was applied in diabetics with and without ESRD (n = 90 for each). Results: Serum MALAT1 expression levels were increased in the ESRD group relative to diabetics without ESRD with median (quartile) values of 10.5 (1.41-126.7) (p < .001). However, miR-499a levels were decreased in more than half of ESRD patients with a median of 0.96 (0.13-3.14). Both MALAT1 and miR-499a expression levels were inversely correlated in the ESRD patient-group.Conclusions: MALAT1 up-regulation and miR-499 down-regulation might be involved in diabetic nephropathy-related ESRD pathogenesis. Functional validation studies are warranted to confirm the MALAT1/miR-499a partnership.
Background: Circulating non-coding RNAs (ncRNAs) have been implicated in health and disease. This study aimed to evaluate the serum expression profile of microRNA-499a (miR-499a) and its selected bioinformatically predicted partner long-ncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) in diabetes-related end-stage renal disease (ESRD) patients and to correlate the expressions with the patients' clinicolaboratory data.Subjects and methods: Real-time quantitative polymerase chain reaction was applied in diabetics with and without ESRD (n = 90 for each). Results: Serum MALAT1 expression levels were increased in the ESRD group relative to diabetics without ESRD with median (quartile) values of 10.5 (1.41-126.7) (p < .001). However, miR-499a levels were decreased in more than half of ESRDpatients with a median of 0.96 (0.13-3.14). Both MALAT1 and miR-499a expression levels were inversely correlated in the ESRDpatient-group.Conclusions: MALAT1 up-regulation and miR-499 down-regulation might be involved in diabetic nephropathy-related ESRD pathogenesis. Functional validation studies are warranted to confirm the MALAT1/miR-499a partnership.
Authors: Eman A Toraih; Ahmed A Abdelghany; Noha M Abd El Fadeal; Essam Al Ageeli; Manal S Fawzy Journal: Graefes Arch Clin Exp Ophthalmol Date: 2019-07-20 Impact factor: 3.117
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Authors: Manal S Fawzy; Essam Al Ageeli; Saeed Awad Al-Qahtani; Baraah T Abu Alsel; Shahad W Kattan; Walla Alelwani; Eman A Toraih Journal: Exp Ther Med Date: 2021-11-22 Impact factor: 2.447
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