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Literature DB >> 3027052

Reduced transposition in rho mutants of Escherichia coli K-12.

Abstract

Substantially reduced frequencies of transposition for the transposons Tn5 and Tn9 and the insertion sequences IS1 and IS5 were observed in several rho mutants of Escherichia coli K-12 compared with those observed in their isogenic wild-type counterparts. The lower transposition frequencies could be due to decreased supercoiling of DNA, to altered expression of required genes, or to aberrant transcription of transposon or target DNA resulting from the lack of transcription termination at Rho-sensitive sites in rho mutants.

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Year: 1987 PMID： 3027052 PMCID： PMC211863 DOI： 10.1128/jb.169.2.888-890.1987

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

30 in total

1. Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.

Authors: A Das; C Merril; S Adhya
Journal: Proc Natl Acad Sci U S A Date: 1978-10 Impact factor: 11.205

2. Reduced superhelicity of plasmid DNA produced by the rho-15 mutation in Escherichia coli.

Authors: J S Fassler; G F Arnold; I Tessman
Journal: Mol Gen Genet Date: 1986-09

3. Termination factor for RNA synthesis.

Authors: J W Roberts
Journal: Nature Date: 1969-12-20 Impact factor: 49.962

4. Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.

Authors: C Egner; D E Berg
Journal: Proc Natl Acad Sci U S A Date: 1981-01 Impact factor: 11.205

5. Three Tn10-associated excision events: relationship to transposition and role of direct and inverted repeats.

Authors: T J Foster; V Lundblad; S Hanley-Way; S M Halling; N Kleckner
Journal: Cell Date: 1981-01 Impact factor: 41.582

6. Distribution of the insertion sequence IS1 in gram-negative bacteria.

Authors: K Nyman; K Nakamura; H Ohtsubo; E Ohtsubo
Journal: Nature Date: 1981-02-12 Impact factor: 49.962

7. An E. coli gene product required for lambda site-specific recombination.

Authors: H I Miller; D I Friedman
Journal: Cell Date: 1980-07 Impact factor: 41.582

8. Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho.

Authors: A Das; D Court; S Adhya
Journal: Proc Natl Acad Sci U S A Date: 1976-06 Impact factor: 11.205

9. The requirement for both DNA polymerase and 5' to 3' exonuclease activities of DNA polymerase I during Tn5 transposition.

Authors: C Sasakawa; Y Uno; M Yoshikawa
Journal: Mol Gen Genet Date: 1981

Reduced transposition in rho mutants of Escherichia coli K-12.

1. Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.

2. Reduced superhelicity of plasmid DNA produced by the rho-15 mutation in Escherichia coli.

3. Termination factor for RNA synthesis.

4. Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.

5. Three Tn10-associated excision events: relationship to transposition and role of direct and inverted repeats.

6. Distribution of the insertion sequence IS1 in gram-negative bacteria.

7. An E. coli gene product required for lambda site-specific recombination.

8. Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho.

9. The requirement for both DNA polymerase and 5' to 3' exonuclease activities of DNA polymerase I during Tn5 transposition.

10. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.

Review 1. Mastering the control of the Rho transcription factor for biotechnological applications.

2. Transcription of the target is required for IS102 mediated deletions.

3. Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase.

4. Target site choice of the related transposable elements Tc1 and Tc3 of Caenorhabditis elegans.

5. A late exclusion of bacteriophage T4 can be suppressed by Escherichia coli GroEL or Rho.

6. Expression of proteins essential for IS1 transposition: specific binding of InsA to the ends of IS1.