Jae-Hyun Jung1, Soo-Byung Park1, Kyung-Hyeon Yoo2, Seog-Young Yoon2, Moon-Kyoung Bae3, Dong Joon Lee4, Ching-Chang Ko4, Yong Hoon Kwon5, Yong-Il Kim6,7. 1. Department of Orthodontics, Dental Research Institute, Pusan National University Dental Hospital, Geumoro 20, Mulgeum, Yangsan, 50612, South Korea. 2. School of Materials Science and Engineering, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan, 46241, South Korea. 3. Department of Oral Physiology, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, South Korea. 4. Department of Orthodontics, School of Dentistry, University of North Carolina, Chapel Hill, NC, 27599, USA. 5. Department of Dental Materials, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, South Korea. y0k0916@pusan.ac.kr. 6. Department of Orthodontics, Dental Research Institute, Pusan National University Dental Hospital, Geumoro 20, Mulgeum, Yangsan, 50612, South Korea. kimyongil@pusan.ac.kr. 7. Institute of Translational Dental Sciences, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, South Korea. kimyongil@pusan.ac.kr.
Abstract
OBJECTIVES: To synthesize two different sizes of bioactive glass-coated mesoporous silica nanoparticles (BGN@MSNs) and to investigate their effects on dentinal tubule occlusion and remineralization. MATERIALS AND METHODS: Two different sizes of mesoporous silica nanoparticles (MSNs) were synthesized using the Stöber method (368A, 1840A) and coated with bioactive glass nanoparticles (BGNs) using a modified quick alkali-mediated sol-gel method (368B, 1840B). Sensitive tooth disc models were prepared and divided into six groups and the following treatments were applied: group 1-no treatment, group 2-bioglass, group 3-368A, group 4-368B, group 5-1840A, and group 6-1840B. Then, five discs were selected from each group and soaked in 6 wt% citric acid to test acid resistance. Dentinal tubule occlusion and occlusion ratio were observed using field-emission scanning electron microscopy. In vitro mineralization tests using simulated body fluid solution were performed to evaluate the remineralization effect of the treatment. RESULTS: All samples effectively occluded the dentinal tubule and formed a membrane-like layer. After acid treatment, 1840B (group 6) exhibited the highest rate of dentinal tubule occlusion. Remineralization was observed in 368B and 1840B, and 1840B exhibited the fastest remineralization. CONCLUSIONS: Dentinal tubule remineralization induced by the BGN@MSN biocomposite can be used to stabilize long-term prognosis in dentin hypersensitivity. The 1840B induced the most efficient remineralization, and its smaller size and larger surface area were effective for remineralization. CLINICAL RELEVANCE: The BGN@MSN biocomposite with its smaller size and larger surface area was more effective for remineralization and dentinal tubule sealing.
OBJECTIVES: To synthesize two different sizes of bioactive glass-coated mesoporous silica nanoparticles (BGN@MSNs) and to investigate their effects on dentinal tubule occlusion and remineralization. MATERIALS AND METHODS: Two different sizes of mesoporous silica nanoparticles (MSNs) were synthesized using the Stöber method (368A, 1840A) and coated with bioactive glass nanoparticles (BGNs) using a modified quick alkali-mediated sol-gel method (368B, 1840B). Sensitive tooth disc models were prepared and divided into six groups and the following treatments were applied: group 1-no treatment, group 2-bioglass, group 3-368A, group 4-368B, group 5-1840A, and group 6-1840B. Then, five discs were selected from each group and soaked in 6 wt% citric acid to test acid resistance. Dentinal tubule occlusion and occlusion ratio were observed using field-emission scanning electron microscopy. In vitro mineralization tests using simulated body fluid solution were performed to evaluate the remineralization effect of the treatment. RESULTS: All samples effectively occluded the dentinal tubule and formed a membrane-like layer. After acid treatment, 1840B (group 6) exhibited the highest rate of dentinal tubule occlusion. Remineralization was observed in 368B and 1840B, and 1840B exhibited the fastest remineralization. CONCLUSIONS: Dentinal tubule remineralization induced by the BGN@MSN biocomposite can be used to stabilize long-term prognosis in dentin hypersensitivity. The 1840B induced the most efficient remineralization, and its smaller size and larger surface area were effective for remineralization. CLINICAL RELEVANCE: The BGN@MSN biocomposite with its smaller size and larger surface area was more effective for remineralization and dentinal tubule sealing.