Chuangxin Liao1, Wenli Chen2, Jinshan Wang2. 1. Department of Neurosurgery, Eastern District of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. Electronic address: lcxsl@126.com. 2. Department of Neurosurgery, Eastern District of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Abstract
OBJECTIVE: MicroRNAs (miRNAs) are a class of small noncoding RNAs that play important roles in tumor development and progression. miR-20a acts as an oncogene in many cancers; however, the underlying role of miR-20a in human glioma remains unknown. METHODS: Glioma tissue samples were obtained from 32 patients with primary glioma who had undergone surgery at the First Affiliated Hospital of Sun Yat-sen University (Guangzhou, China). Twenty-two normal brain tissue samples used as controls were obtained by internal decompression in patients who had undergone surgery for cerebral injury and cerebral hemorrhage at the same hospital. RESULTS: Quantitative reverse transcription polymerase chain reaction showed upregulation of miR-20a in glioma tissues and cell lines compared with normal brain tissue and normal human astrocytes. Functional assays showed that miR-20a promotes proliferation and invasion and inhibits apoptosis in glioma cells. The bioinformatic analysis showed that CELF2 (CUGBP Elav-like family member 2) is a direct target gene of miR-20a, which was confirmed using a luciferase reporter assay. Downregulation of CELF2 reversed the effects of inhibiting miR-20a expression. CONCLUSIONS: Collectively, these results suggest a critical role for miR-20a in glioma cell apoptosis, proliferation, and invasion via the direct targeting of CELF2 and indicate its potential application in cancer therapy.
OBJECTIVE: MicroRNAs (miRNAs) are a class of small noncoding RNAs that play important roles in tumor development and progression. miR-20a acts as an oncogene in many cancers; however, the underlying role of miR-20a in humanglioma remains unknown. METHODS:Glioma tissue samples were obtained from 32 patients with primary glioma who had undergone surgery at the First Affiliated Hospital of Sun Yat-sen University (Guangzhou, China). Twenty-two normal brain tissue samples used as controls were obtained by internal decompression in patients who had undergone surgery for cerebral injury and cerebral hemorrhage at the same hospital. RESULTS: Quantitative reverse transcription polymerase chain reaction showed upregulation of miR-20a in glioma tissues and cell lines compared with normal brain tissue and normal human astrocytes. Functional assays showed that miR-20a promotes proliferation and invasion and inhibits apoptosis in glioma cells. The bioinformatic analysis showed that CELF2 (CUGBP Elav-like family member 2) is a direct target gene of miR-20a, which was confirmed using a luciferase reporter assay. Downregulation of CELF2 reversed the effects of inhibiting miR-20a expression. CONCLUSIONS: Collectively, these results suggest a critical role for miR-20a in glioma cell apoptosis, proliferation, and invasion via the direct targeting of CELF2 and indicate its potential application in cancer therapy.
Authors: Yufen Chen; Amy A Herrold; Zoran Martinovich; Sumra Bari; Nicole L Vike; Anne J Blood; Alexa E Walter; Jaroslaw Harezlak; Peter H Seidenberg; Manish Bhomia; Barbara Knollmann-Ritschel; Khrystyna Stetsiv; James L Reilly; Eric A Nauman; Thomas M Talavage; Linda Papa; Semyon Slobounov; Hans C Breiter Journal: Cereb Cortex Commun Date: 2020-10-26
Authors: Carlos DeOcesano-Pereira; Raquel A C Machado; Ana Marisa Chudzinski-Tavassi; Mari Cleide Sogayar Journal: Int J Mol Sci Date: 2020-04-09 Impact factor: 5.923