Zhenjun Zhu1,2,3, Beiwei Zhu1,2,3, Yujiao Sun1,3,4, Chunqing Ai1,3, Lilong Wang1, Chengrong Wen1,3, Jingfeng Yang1,3, Shuang Song1,3,5, Xiaoling Liu2. 1. School of Food Science and Technology; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian, 116034, China. 2. College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China. 3. National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian, 116034, China. 4. School of Food and Biological Engineering, Shanxi University of Science and Technology, Xi'an, 710021, China. 5. Department of Food Science, University of Massachusetts Amherst, Amherst, MA, 01003, USA.
Abstract
SCOPE: Sulfated polysaccharide from sea cucumber (SCSP), Stichopus japonicus, has been shown to prevent diverse diseases, but little is known about its effects on obesity and gut microbiota in mice fed a high-fat diet (HFD). METHODS AND RESULTS: Diet-induced obese, BALB/c mice are fed an HFD with or without SCSP and depolymerized SCSP (d-SCSP, 0.004 kcal g-1 ) for 8 weeks. The results show that both SCSP and d-SCSP reduce body weight, fat and liver hypertrophy, insulin resistance, and serum lipid and inflammatory cytokine levels in HFD-fed mice. Moreover, SCSP and d-SCSP not only prevent HFD-induced gut disorder, as indicated by the enriched probiotic Akkermansia and reduce endotoxin-bearing Proteobacteria, but also improve the SCFAs and endotoxin (LPS) levels and gut tissue index. Spearman's correlation analysis reveals that several specific genera are strongly correlated with obesity-related indexes. In addition, the inhibitory effect of d-SCSP on fat accumulation is more effective than that of SCSP, which may be related to their different regulation of the gut microbiota. CONCLUSIONS: These findings suggest that SCSP can prevent diet-induced obesity and its associated diseases by modulating the gut microbiota and improving microbial metabolites and gut tissue, and its effects can be enhanced by free-radical depolymerization.
SCOPE: Sulfated polysaccharide from sea cucumber (SCSP), Stichopus japonicus, has been shown to prevent diverse diseases, but little is known about its effects on obesity and gut microbiota in mice fed a high-fat diet (HFD). METHODS AND RESULTS: Diet-induced obese, BALB/c mice are fed an HFD with or without SCSP and depolymerized SCSP (d-SCSP, 0.004 kcal g-1 ) for 8 weeks. The results show that both SCSP and d-SCSP reduce body weight, fat and liver hypertrophy, insulin resistance, and serum lipid and inflammatory cytokine levels in HFD-fed mice. Moreover, SCSP and d-SCSP not only prevent HFD-induced gut disorder, as indicated by the enriched probiotic Akkermansia and reduce endotoxin-bearing Proteobacteria, but also improve the SCFAs and endotoxin (LPS) levels and gut tissue index. Spearman's correlation analysis reveals that several specific genera are strongly correlated with obesity-related indexes. In addition, the inhibitory effect of d-SCSP on fat accumulation is more effective than that of SCSP, which may be related to their different regulation of the gut microbiota. CONCLUSIONS: These findings suggest that SCSP can prevent diet-induced obesity and its associated diseases by modulating the gut microbiota and improving microbial metabolites and gut tissue, and its effects can be enhanced by free-radical depolymerization.
Authors: Irina M Yermak; Aleksandra V Volod'ko; Eleonora I Khasina; Viktoriya N Davydova; Evgeniy A Chusovitin; Dmitry L Goroshko; Anna O Kravchenko; Tamara F Solov'eva; Victor V Maleev Journal: Mar Drugs Date: 2020-05-10 Impact factor: 5.118